Transcript Profile of Transgenic Arabidopsis Constitutively Producing Methyl Jasmonate

Using an Affymetrix GeneChip∨® containing 8300 oligonucleotide probes, we measured transcript levels in transgenic Arabidopsis overexpressing the jasmonate carboxyl methyltransferase gene (AtJMT). When compared with wild-type plants, 5-week-old transgenics exhibited significant alterations (more tha...

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Published in:Journal of plant biology = Singmul Hakhoe chi 2007, 50(1), , pp.12-17
Main Authors: Jung, C.K. (Seoul National University, Seoul, Republic of Korea), Yeu, S.Y. (Seoul National University, Seoul, Republic of Korea), Koo, Y.J. (Seoul National University, Seoul, Republic of Korea), Kim, M.K. (Seoul National University, Seoul, Republic of Korea), Choi, Y.D. (Seoul National University, Seoul, Republic of Korea), Cheong, J.J. (Seoul National University, Seoul, Republic of Korea), E-mail: cheongjj@snu.ac.kr
Format: Article
Language:English
Subjects:
gene transcript profile
jasmonic acid methyltransferase
METHYL JASMONATE
METIL JASMONATO
microarray
transgenic Arabidopsis
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Summary:Using an Affymetrix GeneChip∨® containing 8300 oligonucleotide probes, we measured transcript levels in transgenic Arabidopsis overexpressing the jasmonate carboxyl methyltransferase gene (AtJMT). When compared with wild-type plants, 5-week-old transgenics exhibited significant alterations (more than a two-fold increase or decrease) in the expression levels of 168 genes. Among them, 80 were up-regulated, including those involved in defense, oxidative stress-tolerance, and senescence. In contrast, the expression of 88 genes, including those that function in photosynthesis and cold/drought-stress responses, was significantly down-regulated.
ISSN:1226-9239
1867-0725
DOI:10.1007/BF03030594