Isolation and Characterization of MADS Box Genes Possibly Related to Root Development in Sweetpotato (Ipomoea batatas L. Lam.)

Development of the tuberous root of sweetpotato coincides with abnormal vascular cambium activity. The genes and pathways predominately active for vascular morphogenesis in sweetpotato root are unknown. In this study, using a reverse transcription-polymerase chain reaction (RT-PCR) approach, we foun...

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Bibliographic Details
Published in:Journal of plant biology = Singmul Hakhoe chi 2005, 48(4), , pp.387-393
Main Authors: Sun Hyung Kim, Tatsuro Hamada, Matoyasu Otani, Takiko Shimada
Format: Article
Language:Korean
Subjects:
IbMADS79 clade
MADS box gene
root development
sweetpotato
생물학
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Summary:Development of the tuberous root of sweetpotato coincides with abnormal vascular cambium activity. The genes and pathways predominately active for vascular morphogenesis in sweetpotato root are unknown. In this study, using a reverse transcription-polymerase chain reaction (RT-PCR) approach, we found three sweetpotato MADS box genes expressed in root tissues./bAGL17 is an AGL17 clade MADS box gene, and was mainly expressed in tuberous root as demonstrated by RT-PCR analysis. IbAGL20 is a TM3-like gene that is expressed at similar levels in leaf, vegetative shoot, flower and root tissues. IbMADS79 closely resembled the Arabidopsis API and Antirrhinum SQUA genes, but /bMADS79 was expressed only in roots, whereas the mRNA levels of genes of SQUA clade were high in the flower and floral meristem. RT-PCR results confirmed that MADS box genes were also expressed in different developmental stages. These differentially expressed MADS box genes will be potential candidates for research to elucidate the molecular process related to root development in sweetpotato.
ISSN:1226-9239
1867-0725