Isolation and Characterization of Squalene Synthase cDNA from Centella asiatica (L.) Urban

We have cloned and characterized a gene for squalene synthase (SQS) from Centella asiatica (L.) Urban, a species that produces a large quantity of triterpene saponins such as asiaticoside and madecassoside. Its full-length cDNA clone was isolated by RACE PCR. The sequence of pSQS contains an open re...

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Bibliographic Details
Published in:Journal of plant biology = Singmul Hakhoe chi 2005, 48(3), , pp.263-269
Main Authors: 황백, 성낙술, 김민영, 김옥태
Format: Article
Language:English
Subjects:
생물학
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Summary:We have cloned and characterized a gene for squalene synthase (SQS) from Centella asiatica (L.) Urban, a species that produces a large quantity of triterpene saponins such as asiaticoside and madecassoside. Its full-length cDNA clone was isolated by RACE PCR. The sequence of pSQS contains an open reading frame of 1248 nucleotides, which code for 416 amino acids with a molecular mass of 47.3 kDa. Southern analysis revealed that one copy might exist in the C. asiatica genome. We also determined that 0.1 mM methyl jasmonate was sufficient to up-regulate those levels of CaSQS mRNA. KCI Citation Count: 27
ISSN:1226-9239
1867-0725
DOI:10.1007/BF03030521