Response surface methodology based optimization of keratinase production from alkali-treated feather waste and horn waste using Bacillus sp. MG-MASC-BT

A native feather-degrading keratinolytic bacterium, Bacillus sp. MG-MASC-BT was isolated from feather dumping soil in Mallasamudram, Tamil Nadu, India and screened for keratinase production using alkali treated horn waste (HW) and feather waste (FW). The study factors influencing keratinase producti...

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Bibliographic Details
Published in:Journal of industrial and engineering chemistry (Seoul, Korea) 2015, 27(0), , pp.25-30
Main Authors: Govarthanan, Muthusamy, Selvankumar, Thangasamy, Selvam, Kandasamy, Sudhakar, Chinnappan, Aroulmoji, Vincent, Kamala-Kannan, Seralathan
Format: Article
Language:English
Subjects:
Bacillus sp
Feather
Horn
Keratinase
Life Sciences
Solid state fermentation
화학공학
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Summary:A native feather-degrading keratinolytic bacterium, Bacillus sp. MG-MASC-BT was isolated from feather dumping soil in Mallasamudram, Tamil Nadu, India and screened for keratinase production using alkali treated horn waste (HW) and feather waste (FW). The study factors influencing keratinase production was optimized by Box–Behnken design (BBD). The maximum enzyme production (1075U/ml) was observed at pH 7.0, temperature 55°C and growth period of 60h. The media supplemented with 6% of HW and FW enhanced keratinase production. Statistics based contour plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and the culture conditions.
ISSN:1226-086X
1876-794X
DOI:10.1016/j.jiec.2014.12.022