Preparation of phosphonated gelatin-coated titanium containing rhBMP-2 by UV irradiation for improved osteoinduction and function

Gelatin derivative was prepared to coating titanium surface and photo-immobilize rhBMP-2 for improved osteoinduction and function. The conjugation was confirmed by 31P and 1H NMR. Photo-immobilization of the azide–phosphonate–gelatin (Az–P–Gel) was evaluated by micropatterning. The rate of recombina...

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Bibliographic Details
Published in:Journal of industrial and engineering chemistry (Seoul, Korea) 2016, 36(0), , pp.66-73
Main Authors: Heo, Yun, Kim, Eun-Hye, Kobatake, Eiry, Nah, Jae-Woon, Ito, Yoshihiro, Son, Tae-Il
Format: Article
Language:English
Subjects:
Assaying
Biocompatibility
Biomedical materials
Bones
Coating
Contact angle
Drug-delivery system
Gelatin
Osteoinduction
Photo-immobilization
Recombinant
Titanium
Titanium surface modification
화학공학
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Summary:Gelatin derivative was prepared to coating titanium surface and photo-immobilize rhBMP-2 for improved osteoinduction and function. The conjugation was confirmed by 31P and 1H NMR. Photo-immobilization of the azide–phosphonate–gelatin (Az–P–Gel) was evaluated by micropatterning. The rate of recombinant human bone morphogenetic protein (rhBMP-2) release was evaluated for performance of Az–P–Gel as a carrier. The water contact angle was determined to characterize Ti surface coated with Az–P–Gel. Ability to induce osteogenesis of Az–P–Gel was evaluated using alkaline phosphatase (ALP) activity assay, Alizarin Red S staining, and calcium colorimetric assay. Our results demonstrate that Ti modified with Az–P–Gel and rhBMP-2 facilitates bone regeneration.
ISSN:1226-086X
1876-794X
DOI:10.1016/j.jiec.2016.01.006