Colorimetric Determination of Singlet Oxygen Scavengers Using a Protein Photosensitizer

Despite the diverse roles of singlet oxygen ( 1 O 2 ) in plants and animals, simple detection of 1 O 2 has been limited due to technical difficulties. Here we report a colorimetric method for visualizing the generation and suppression of 1 O 2 using a protein photo-sensitizer, mini singlet oxygen ge...

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Bibliographic Details
Published in:Biochip journal 2020, 14(2), , pp.148-157
Main Authors: Kim, Miso, Kim, Eun Hye, Pham, Thi Hai Yen, Le, Tuan Anh Hoang, Do, Thi Phuong, Nguyen, Thi Nga, Nguyen, Thi Cuc, Trieu, Ha Phuong, Do, Thi Thao, Kim, Young-Pil
Format: Article
Language:English
Subjects:
Antioxidants
Biomedical Engineering and Bioengineering
Biotechnology
Chemistry
Colorimetry
Light
Light irradiation
Original Article
Oxidants
Oxidizing agents
Proteins
Reactive oxygen species
Reagents
Scavengers
Scavenging
Singlet oxygen
Standard deviation
생물공학
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Summary:Despite the diverse roles of singlet oxygen ( 1 O 2 ) in plants and animals, simple detection of 1 O 2 has been limited due to technical difficulties. Here we report a colorimetric method for visualizing the generation and suppression of 1 O 2 using a protein photo-sensitizer, mini singlet oxygen generator (miniSOG). Upon external light irradiation, miniSOG strongly produced 1 O 2 and oxidized a universal chromogenic reagent, tetramethylbenzidine, leading to a rapid color change in solution. We used this finding to demonstrate the 1 O 2 scavenging effects of well-known anti-oxidants and of natural compounds extracted from plants. Owing to its high usability and simplicity, our developed assay platform will be useful for screening potential scavengers for reactive oxygen species and will promote the development of various antioxidants.
ISSN:1976-0280
2092-7843
DOI:10.1007/s13206-019-4203-6