Effects of the DNA repair inhibitors, cytosine arabinoside and 3-aminobenzamide, on the frequency of radiation-induced micronuclei, nuclear buds, and nucleoplasmic bridges

Background Micronuclei (MN), nuclear bud (NBud), and nucleoplasmic bridge (NPB) are suggested as biomarkers for radiation exposure; however, they have not been extensively studied to understand the underlying mechanisms responsible for their formation. Objectives To (1) validate NBud and NPB within...

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Published in:Genes & genomics 2020, 42(6), , pp.673-680
Main Authors: Cho, Yoon Hee, Kim, Yang Jee, Lee, Sunyeong, Joung, Kyung-in, Chung, Hai Won, Kim, Sunmi, Kim, Su Young
Format: Article
Language:English
Subjects:
Adult
Animal Genetics and Genomics
Benzamides - pharmacology
Biomarkers
Biomedical and Life Sciences
Cells, Cultured
Chromosomes
Cytarabine
Cytarabine - pharmacology
Cytokinesis
Cytosine
Deoxyribonucleic acid
DNA
DNA repair
DNA Repair - drug effects
Female
Human Genetics
Humans
Life Sciences
Microbial Genetics and Genomics
Micronuclei
Micronuclei, Chromosome-Defective - drug effects
Micronuclei, Chromosome-Defective - radiation effects
Plant Genetics and Genomics
Radiation Tolerance
Research Article
X-Rays
생물학
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Summary:Background Micronuclei (MN), nuclear bud (NBud), and nucleoplasmic bridge (NPB) are suggested as biomarkers for radiation exposure; however, they have not been extensively studied to understand the underlying mechanisms responsible for their formation. Objectives To (1) validate NBud and NPB within the cytokinesis-blocked micronucleus (CBMN) assay as biomarkers for radiation exposure and (2) determine the effects of the DNA repair inhibitors, cytosine arabinoside (Ara C) and 3-aminobenzamide (3-AB) on radiation-induced MN, NBud, and NPB formation. Methods Human blood samples were irradiated with 0–3 Gy X-rays and subsequently treated with Ara C and 3-AB. CBMN and chromosome aberration assays were carried out to measure MN, NBud, and NPB and dicentric chromosomes, respectively. Results The frequency of radiation-induced MN, NBud, and NPB increased in a dose-dependent manner. The frequency of MN, NBud, and NPB was highly and positively correlated with the dicentric chromosome, a standard biomarker for biodosimetry ( r  > 0.98, p  
ISSN:1976-9571
2092-9293
DOI:10.1007/s13258-020-00934-8