Viscozyme L aided flavonoid extraction and identification of quercetin from Saururus chinensis (Lour.) Baill

In order to enhance the extraction efficiency of flavonoid from Saururus chinensis, carbohydrate-hydrolyzing enzyme Viscozyme L aided extraction techniques have been studied. Then flavonoid composition, as well as quercetin, were also identified using UV/Vis, HPLC/MS, and 1H-NMR. The results showed...

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Bibliographic Details
Published in:Journal of applied biological chemistry 2020, 63(3), , pp.197-201
Main Authors: Zheng, Hu-Zhe, Kwon, Sun-Young, Chung, Shin-Kyo
Format: Article
Language:English
Subjects:
농학
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Summary:In order to enhance the extraction efficiency of flavonoid from Saururus chinensis, carbohydrate-hydrolyzing enzyme Viscozyme L aided extraction techniques have been studied. Then flavonoid composition, as well as quercetin, were also identified using UV/Vis, HPLC/MS, and 1H-NMR. The results showed that favorable extraction conditions were Viscozyme L concentration of 0.25 mg/g, pH 4.2, reaction at 45 oC for 12 h. Under the favorable extraction condition, total flavonoid yield (37.9 mg/g) and quercetin yield (0.86 mg/g) increased by about 2.0 and 9.6 times, respectively, compared to control group. Interestingly, as a significant flavonoid of S. chinensis, flavonoid glycones rutin was hydrolyzed to aglycones quercetin by Viscozyme L. These findings provide scientific and theoretical support for the development quercetin-rich products, which was quickly absorbed by the human body than rutin. KCI Citation Count: 0
ISSN:1976-0442
2234-7941
DOI:10.3839/jabc.2020.027