Evaluation of Hyaluronic Acid/Agarose Hydrogel for Cartilage Tissue Engineering Biomaterial

Hyaluronic acid (HA) is one of the most applied biomaterials in a tissue engineering field due to its biocompatibility and its presence in the native extracellular matrix (ECM) of tissues. However, the mechanical property of the HA is weak and requires specific treatment to improve its properties. T...

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Bibliographic Details
Published in:Macromolecular research 2020, 28(11), , pp.979-985
Main Authors: Choi, Joo Hee, Kim, Jin Su, Kim, Won Kyung, Lee, Wonchan, Kim, Namyeong, Song, Cheol Ui, Jung, Jun Jae, Song, Jeong Eun, Khang, Gilson
Format: Article
Language:English
Subjects:
Biocompatibility
Biomedical materials
Cartilage
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Complex Fluids and Microfluidics
Compressive strength
Hyaluronic acid
Hydrogels
Mechanical properties
Nanochemistry
Nanotechnology
Physical Chemistry
Polymer Sciences
Soft and Granular Matter
Swelling ratio
Tissue engineering
Weight loss
고분자공학
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Summary:Hyaluronic acid (HA) is one of the most applied biomaterials in a tissue engineering field due to its biocompatibility and its presence in the native extracellular matrix (ECM) of tissues. However, the mechanical property of the HA is weak and requires specific treatment to improve its properties. The application of Agarose (AG) hydrogel is widely studied and used as a support for the three-dimensional culture of cells due to its biocompatibility. Nevertheless, AG itself lacks the biological environment of the matrix which is unsuitable for the growth of the encapsulated cells. In this study, the composite of HA hydrogel and AG hydrogel (HA/AG hydrogel) is proposed to supplement the drawbacks of each hydrogel. HA provided enhanced microenvironment of matrix and AG improved the mechanical properties and assisted the cells. The characterization of the blended hydrogels was carried out with FT-IR, weight loss, swelling ratio, and compressive strength study. The biocompatibility and biological environment of the composite was evaluated by dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide; thiazolyl blue (MTT), live/dead staining, and morphological study. The composite biomaterial exhibited applicability for cartilage tissue engineering and the in vitro study of the cell-laden HA/AG hydrogel displayed potential for cartilage tissue engineering in the future.
ISSN:1598-5032
2092-7673
DOI:10.1007/s13233-020-8137-6