Detecting Salmonella Type II flagella production by transmission electron microscopy and immunocytochemistry

The bacterial flagellum is an appendage structure that provides a means for motility to promote survival in fluctuating environments. For the intracellular pathogen Salmonella enterica serovar Typhimurium to survive within macrophages, flagellar gene expression must be tightly regulated, and thus, i...

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Bibliographic Details
Published in:The journal of microbiology 2020, 58(4), , pp.245-251
Main Authors: Han, Yoontak, Lee, Eun-Jin
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Deoxyribonucleic acid
DNA
Electron microscopy
Flagella
Gene expression
Immunocytochemistry
Life Sciences
Macrophages
Microbiology
Microscopy
Pathogenesis
Post-transcription
Protein biosynthesis
Protein synthesis
Protocol
Recombination
Salmonella
Survival
Transcription
Transmission electron microscopy
생물학
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Summary:The bacterial flagellum is an appendage structure that provides a means for motility to promote survival in fluctuating environments. For the intracellular pathogen Salmonella enterica serovar Typhimurium to survive within macrophages, flagellar gene expression must be tightly regulated, and thus, is controlled at multiple levels, including DNA recombination, transcription, post-transcription, protein synthesis, and assembly within host cells. To understand the contribution of flagella to Salmonella pathogenesis within the host, it is critical to detect flagella production within macrophages via microscopy. In this paper, we describe two methods for detecting bacterial flagella by microscopy both in vitro and in vivo infection models.
ISSN:1225-8873
1976-3794
DOI:10.1007/s12275-020-9297-y