Cyclodextrin-mediated gold nanoparticles as multisensing probe for the selective detection of hydroxychloroquine drug

• Published in: The Korean journal of chemical engineering 2021, 38(3), 252, pp.624-634
• Main Authors: George, Jaise Mariya, Mathew, Beena
• Format: Article
• Language: English
• Subjects: Biotechnology; Catalysis; Chemistry; Chemistry and Materials Science; Cyclodextrins; Doppler effect; Electronic; Enantiomers; Fluorescence; Gold; Industrial Chemistry/Chemical Engineering; Inorganic; Materials (Organic; Materials (Organic, Inorganic, Electronic, Thin Films); Materials Science; Nanoparticles; Photon correlation spectroscopy; Red shift; Selectivity; Surface plasmon resonance; Thin Films; Zeta potential; 화학공학
• ISSN: 0256-1115; 1975-7220
• DOI: 10.1007/s11814-020-0719-7

β -Cyclodextrin ( β -CD) modified gold nanoparticles (AuNP) were rapidly synthesized using microwave assisted procedure. Parameters, such as time, pH and concentrations of β -CD and gold, were optimized for the synthesis of β -CD-AuNP. The addition of enantiomers and racemic mixture of hydroxychloroquine (R-HCQ, S-HCQ and RS-HCQ) drugs and their interaction with β -CD led to a red shift in the surface plasmon resonance of β -CD-AuNP. The changes associated with the introduction of HCQ in β -CD-AuNP were studied using various characterization techniques such as UV-vis, FT-IR, XRD, dynamic light scattering, zeta potential, transmission electron microscopy, fluorescence spectroscopy and electrochemical techniques. The host-guest interaction of β -cyclodextrin with S-HCQ, R-HCQ and RS-HCQ resulted in the aggregation of gold nanoparticles. The surface plasmon resonance at 521 nm for β -CD-AuNP was shifted to 600, 620 and 670 nm on the addition of S-HCQ, R-HCQ and RS-HCQ, respectively, with a color change from pink to blue. The selectivity and sensitivity of the developed system for RS-HCQ were investigated and the limit of detection (LOD=3 s/m) was found to be 2.61, 0.15, and 0.85 nM for optical, fluorescence and electrochemical methods, respectively. The successful monitoring of RS-HCQ drug in pharmaceutical samples is possible with these techniques.