Expression of TAS2R14 in the intestinal endocrine cells of non-human primates

• Published in: Genes & genomics 2021, 43(3), , pp.259-267
• Main Authors: Hayashi, Misa, Inaba, Akihiko, Hakukawa, Miho, Iwatsuki, Ken, Imai, Hiroo, Masuda, Katsuyoshi
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; Animals; Biomedical and Life Sciences; Bitter taste; Cholecystokinin; Chromosome 16; Colon; Digestive system; Duodenum; Enteroendocrine Cells - metabolism; Gastrointestinal tract; HEK293 Cells; Human Genetics; Humans; Ileum; Immunoreactivity; Intestine; Intestines - metabolism; Life Sciences; Macaca mulatta; Microbial Genetics and Genomics; Monkeys & apes; Papillae; Plant Genetics and Genomics; Primates; Receptors, G-Protein-Coupled - genetics; Receptors, G-Protein-Coupled - metabolism; Research Article; RNA, Messenger - metabolism; Serotonin; Taste receptors; 생물학
• ISSN: 1976-9571; 2092-9293
• DOI: 10.1007/s13258-021-01054-7

Background Recent studies have demonstrated that genes related to bitter taste receptors (TAS2Rs) on various chromosomes are expressed in extra-oral organs of various animals. The bitter taste receptor TAS2R14 is conserved among primate species and shows broad ligand sensitivity. Mice have a number of orthologues to primate TAS2R14 located in tandem on chromosome 16; however, their expression patterns are not unique. Objective We characterized the expression of TAS2R14 in various cell types in the intestines of the rhesus macaque and evaluated its role in hormone production in the gut. Methods TAS2R14 expression was examined in the intestines of rhesus macaques, a common non-human primate model, by RT-qPCR and immunohistochemical staining. Results Mean expression levels of TAS2R14 in the duodenum, ileum, and colon were similar to each other and were lower than those in circumvallate papillae. An immunohistochemical analysis revealed TAS2R14 immunoreactivity in enteroendocrine cells positive for cholecystokinin, serotonin, and the G protein GNAT3. Conclusion These results suggest that primate TAS2R14 is broadly expressed in the intestine, mainly in enteroendocrine cells, and promotes gut hormone secretion in response to bitter stimuli.