Development and validation of multiplex PCR assay for differentiating tunas and billfishes

Commercially available tunas and billfishes are generally processed as steaks, making it difficult to visually distinguish between the two. We developed and validated species-specific primers to prevent the adulteration of tunas by billfishes. Tunas and billfishes primers were designed on the cytoch...

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Bibliographic Details
Published in:Food science and biotechnology 2021, 30(4), , pp.497-503
Main Authors: Kim, Na-Ye-Seul, Park, Eun-Ji, Lee, Seo-Hyun, Mun, Kwang-Ho, Yang, Ji-Young, Kim, Jung-Beom
Format: Article
Language:English
Subjects:
Chemistry
Chemistry and Materials Science
Cytochrome
Cytochromes
Food Science
Multiplexing
Nutrition
Primers
Product names
Research Article
식품과학
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Summary:Commercially available tunas and billfishes are generally processed as steaks, making it difficult to visually distinguish between the two. We developed and validated species-specific primers to prevent the adulteration of tunas by billfishes. Tunas and billfishes primers were designed on the cytochrome oxidase subunit I. Multiplex PCR bands obtained were 579 bp, 291 bp and 114 bp for tunas, billfishes and internal control. Sensitivity was determined to be 5 ng for tunas and billfishes. A total of 50 samples were monitored: 49 for tunas and 1 for billfish. As a result of the monitoring, the fake tunas did not show due to the agreement between product name and the raw material of the wrapping paper. Our results indicate that the species-specific primers developed in this study are suitable for differentiating tunas and billfishes. The newly developed multiplex PCR assay is a time and cost effective technique for determining the authenticity of tunas and billfishes.
ISSN:1226-7708
2092-6456
DOI:10.1007/s10068-021-00893-0