Complete mitochondrial genome of the hemp borer, Grapholita delineana (Lepidoptera: Tortricidae): Gene variability and phylogeny among Grapholita

• Published in: Journal of Asia-Pacific entomology 2021, 24(2), , pp.250-258
• Main Authors: Song, Lu, Shi, Yuxia, Zhang, Hongfei, Wang, Zhengbing, Liu, Xiaomeng, Yang, Mingsheng
• Format: Article
• Language: English
• Subjects: Mitogenome; Pest management; Phylogenomics; Tortricidae; Tortricoidea; 농학
• ISSN: 1226-8615; 1876-7990
• DOI: 10.1016/j.aspen.2021.02.002

[Display omitted] •Complete mitogenome of Grapholita delineana was provided by NGS.•nad6 exhibits the highest evolutionary rate among 13 protein-coding genes.•The motif ‘ATACTAA’ is rarely observed at 3′ end of nad1.•Phylogenetic analyses reveal the non-monophyly of Grapholita. The mitochondrial genome (mitogenome) has been extensively used in phylogenetics and species-level evolutionary investigations. The lepidopteran family Tortricidae (leaf-roller moths), including the genus Grapholita, contains numerous species of economic importance, but for the majority of Grapholita species, their mitogenomes remain poorly studied. Here, we sequence and annotate the full mitogenome of Grapholita delineana, an important pest of hemp worldwide and compare it with the mitogenomes of two congeneric species available from GenBank. The G. delineana mitogenome is 15,599 bp long, including 37 typical mitochondrial genes and an A + T-rich region. Gene content, order and orientation are identical to other reported tortricid mitogenomes. Analyses of nucleotide diversity, Ka/Ks, genetic distance and number of variable sites together suggest that nad6 is the fastest-evolving gene among the mitochondrial PCGs of Grapholita. Our analyses indicate that Grapholita, as presently defined, is not monophyletic, confirming previous morphological and multiple-gene studies, using mitogenomic evidence. Our study provides information on comparative mitogenomics of Tortricidae especially Grapholita.