Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine

The objective of the present study was to evaluate the immune-enhancing potential of outer membrane protein (OMP) and alum as adjuvants towards inactivated Vero cells rabies vaccine (FRV/K2). Six groups of female Sprague Dawley albino rats (10/group) were used in the evaluation of immunogenicity and...

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Published in:Clinical and experimental vaccine research (Seoul) 2021, 10(2), , pp.132-140
Main Authors: Negm, Iman Ibrahim, Ragab, Yasser M, Mohamed, Aly Fahmy
Format: Article
Language:English
Subjects:
Aluminum
Antibodies
Antigens
Ethics
Immunoglobulins
Laboratory animals
Ligands
Lymphocytes
Original
Proteins
R&D
Rabies
Research & development
Salmonella
Vaccines
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Summary:The objective of the present study was to evaluate the immune-enhancing potential of outer membrane protein (OMP) and alum as adjuvants towards inactivated Vero cells rabies vaccine (FRV/K2). Six groups of female Sprague Dawley albino rats (10/group) were used in the evaluation of immunogenicity and safety of vaccines and adjuvants. Total immunoglobulin G secreted interferon-gamma (IFN-γ), and the percentage of proliferated CD4+ and CD8+ T cells were measured. Biochemical analysis and histopathological examination were used to test safety profiles. OMP adjuvanted rabies vaccine (FRV/K2+OMP) (OMP combined locally prepared vaccine) induced significantly higher neutralizing antibodies on day 21 post-vaccination relative to free (FRV/K2) vaccine and alum adsorbed vaccine (FRV/K2+alum) (alum adsorbed locally prepared vaccine). (FRV/K2+OMP) induced a significantly higher level of IFN-γ on day 14 post-vaccination. CD8+ T cells were significantly higher post-vaccination with reference (RV), free (FRV/K2), and (FRV/K2+OMP) than (FRV/K2+alum). On the contrary, CD4+ T cells were significantly elevated post-vaccination with (FRV/K2+alum) at p
ISSN:2287-3651
2287-366X
DOI:10.7774/CEVR.2021.10.2.132