Anti-SARS-CoV-2 Nucleoprotein Antibodies Derived from Pig Serum with a Controlled Specificity

Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (NP) were purified from pig serum through two steps: (1) isolation of anti-NP IgG antibodies using magnetic beads with immobilized human SARS-CoV-2 NP and (2) filtration of anti-spike protein (SP) IgG antib...

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Bibliographic Details
Published in:Biochip journal 2021, 15(2), , pp.195-203
Main Authors: Jung, Jaeyong, Bong, Ji-Hong, Kim, Hong-Rae, Park, Jun-Hee, Lee, Chang Kyu, Kang, Min-Jung, Kim, Hyun Ok, Pyun, Jae-Chul
Format: Article
Language:English
Subjects:
Antibodies
Biomedical Engineering and Bioengineering
Biosensors
Biotechnology
Chemistry
Chemistry and Materials Science
Competition
Coronaviruses
Filtration
Hogs
Immunoassay
Immunoglobulin G
Middle East respiratory syndrome
Original Article
Proteins
Severe acute respiratory syndrome coronavirus 2
Spike protein
Surface plasmon resonance
Viral diseases
생물공학
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Summary:Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (NP) were purified from pig serum through two steps: (1) isolation of anti-NP IgG antibodies using magnetic beads with immobilized human SARS-CoV-2 NP and (2) filtration of anti-spike protein (SP) IgG antibodies using magnetic beads with immobilized human SARS-CoV SP. The enhanced specificity of the purified antibodies to the NP of SARS-CoV-2 was demonstrated using an immunoassay with anti-NP IgG antibodies after the isolation and filtration steps. The binding constants ( K d ) of the purified anti-NP IgG antibodies to the NP of SARS-CoV-2 and the SP of SARS-CoV were estimated using a surface plasmon resonance biosensor (SPR). A competitive assay using the two-step purified anti-NP IgG antibodies from pig serum demonstrated (a) the detection of SARS-CoV-2 in viral fluid and (b) the discrimination of SARS-CoV-2 from SARS-CoV, MERS-CoV, and CoV strain 229E in viral fluids.
ISSN:1976-0280
2092-7843
DOI:10.1007/s13206-021-00019-y