Flow cytometric monitoring of the bacterial phenotypic diversity in aquatic ecosystems

Flow cytometry is a promising tool used to identify the phenotypic features of bacterial communities in aquatic ecosystems by measuring the physical and chemical properties of cells based on their light scattering behavior and fluorescence. Compared to molecular or culture-based approaches, flow cyt...

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Bibliographic Details
Published in:The journal of microbiology 2021, 59(10), , pp.879-885
Main Authors: Hong, Jin-Kyung, Kim, Soo Bin, Ahn, Seok Hyun, Choi, Yongjoo, Lee, Tae Kwon
Format: Article
Language:English
Subjects:
Aquatic ecosystems
Bacteria
Bacteria - classification
Bacteria - cytology
Bacteria - genetics
Bacteria - isolation & purification
Biomedical and Life Sciences
Cell culture
Chemical properties
Chemicophysical properties
DNA, Bacterial - genetics
Ecosystem
Ecosystems
Flow cytometry
Flow Cytometry - methods
Fluorescence
Gene sequencing
Groundwater - microbiology
Life Sciences
Light scattering
Microbiology
Microorganisms
Monitoring
Phenotype
Protocol
RNA, Ribosomal, 16S - genetics
Sample preparation
Statistical analysis
Water Microbiology
Water quality
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Summary:Flow cytometry is a promising tool used to identify the phenotypic features of bacterial communities in aquatic ecosystems by measuring the physical and chemical properties of cells based on their light scattering behavior and fluorescence. Compared to molecular or culture-based approaches, flow cytometry is suitable for the online monitoring of microbial water quality because of its relatively simple sample preparation process, rapid analysis time, and high-resolution phenotypic data. Advanced statistical techniques (e.g., denoising and binning) can be utilized to successfully calculate phenotypic diversity by processing the scatter data obtained from flow cytometry. These phenotypic diversities were well correlated with taxonomic-based diversity computed using next-generation 16S RNA gene sequencing. The protocol provided in this paper should be a useful guide for a fast and reliable flow cytometric monitoring of bacterial phenotypic diversity in aquatic ecosystems.
ISSN:1225-8873
1976-3794
DOI:10.1007/s12275-021-1443-7