Description of Ornithinimicrobium ciconiae sp. nov., and Ornithinimicrobium avium sp. nov., isolated from the faeces of the endangered and near-threatened birds

• Published in: The journal of microbiology 2021, 59(11), , pp.978-987
• Main Authors: Lee, So-Yeon, Sung, Hojun, Kim, Pil Soo, Kim, Hyun Sik, Lee, Jae-Yun, Lee, June-Young, Jeong, Yun-Seok, Tak, Euon Jung, Han, Jeong Eun, Hyun, Dong-Wook, Bae, Jin-Woo
• Format: Article
• Language: English
• Subjects: Actinobacteria - classification; Actinobacteria - genetics; Actinobacteria - isolation & purification; Actinobacteria - metabolism; Aerobes; Alanine; Amino acids; Animals; Aquatic birds; Aspartic acid; Bacterial Typing Techniques; Biomedical and Life Sciences; Birds - classification; Birds - microbiology; Cardiolipin; DNA, Bacterial - genetics; Endangered Species; Faeces; Fatty acids; Fatty Acids - chemistry; Fatty Acids - metabolism; Feces; Feces - microbiology; Gene sequencing; Genomes; Genomic analysis; Glycine; Glycine (amino acid); L-Alanine; Life Sciences; Lipids; Microbial Systematics and Evolutionary Microbiology; Microbiology; New species; Nucleotides; Ornithine; pH effects; Phosphatidylglycerol; Phylogeny; Quinones; Ribose; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequencing; Similarity; Sodium chloride; Spores; Sugar; Waterfowl; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-021-1323-1

Phenotypic and genomic analyses were performed to characterize two novel species, H23M54 T and AMA3305 T , isolated from the faeces of the Oriental stork ( Ciconia boyciana ) and the cinereous vulture ( Aegypius monachus ), respectively. Strains H23M54 T and AMA3305 T showed the highest similarities of 16S rRNA gene sequences and complete genome sequences with Ornithinimicrobium cavernae CFH 30183 T (98.5% of 16S rRNA gene sequence similarity and 82.1% of average nucleotide identity, ANI) and O. pekingense DSM 21552 T (98.5% of 16S rRNA gene sequence similarity and 82.3% of ANI), respectively. Both strains were Gram-stain-positive, obligate aerobes, non-motile, non-spore-forming, and coccoid- and rodshaped. Strain H23M54 T grew optimally at 25–30°C and pH 8.0 and in the presence of 1.5–2% (wt/vol) NaCl, while strain AMA3305 T grew optimally at 30°C and pH 7.0 and in the presence of 1–3% (wt/vol) NaCl. Both strains had iso-C 15:0 , iso-C 16:0 , and summed feature 9 (iso-C 17:1 ω 9 c and/or C 16:0 10-methyl) as major cellular fatty acids. MK-8 (H 4 ) was identified as the primary respiratory quinone in both strains. Strains H23M54 T and AMA3305 T possessed diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. Moreover, strains H23M54 T and AMA3305 T commonly contained ribose and glucose as major sugars and l -ornithine, l -alanine, glycine, and aspartic acid as major amino acids. The polyphasic taxonomic data indicate that strains H23M54 T and AMA3305 T represent novel species of the genus Ornithinimicrobium. We propose the names Ornithinimicrobium ciconiae sp. nov. and Ornithinimicrobium avium sp. nov. for strains H23M54 T (= KCTC 49151 T = JCM 33221 T ) and AMA3305 T (= KCTC 49180 T = JCM 32873 T ), respectively.