AtLRRop2, an leucine-rich repeat-only protein, mediates cold stress response in Arabidopsis thaliana

Cold stress is one of the main abiotic stresses that cause major damage to plant growth and development. Plants activate a number of response mechanisms to increase tolerance to environmental stresses. To investigate the important components of cold stress signaling in plants, we identified a novel...

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Bibliographic Details
Published in:Plant biotechnology reports 2021, 15(5), , pp.641-649
Main Authors: Park, Hyeong Cheol, Kim, Dong Wook, Park, Jiyoung, Baek, Dongwon, Yun, Dae-Jin
Format: Article
Language:English
Subjects:
Agriculture
Amino acid sequence
Amino acids
Arabidopsis thaliana
Biomedical and Life Sciences
Biotechnology
Cell Biology
Cold
Cold tolerance
Environmental stress
Freezing
Leucine
Life Sciences
Mutants
Original Article
Plant Biochemistry
Plant growth
Plant Sciences
Polymerase chain reaction
Protein interaction
Proteins
Reverse transcription
Sequence analysis
Signaling
Stresses
T-DNA
농학
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Summary:Cold stress is one of the main abiotic stresses that cause major damage to plant growth and development. Plants activate a number of response mechanisms to increase tolerance to environmental stresses. To investigate the important components of cold stress signaling in plants, we identified a novel Arabidopsis thaliana gene, which encodes an leucine-rich repeat-only protein, AtLRRop2 ( At2g20210 ), and demonstrated its role in the cold stress response. Amino acid sequence analysis of AtLRRop2 revealed the presence of four leucine-rich repeat (LRR) motifs that mediate protein–protein interactions. To determine the function of AtLRRop2 in cold stress response, we identified three independent T-DNA insertion mutants, atlrrop2-1 , atlrrop2-2 , and atlrrop2-3 , and examined the transcript levels by reverse transcription PCR (RT-PCR). The results revealed that atlrrop2-1 and atlrrop2-2 are knockout mutants, while atlrrop2-3 is a knockdown mutant. All three mutants were more sensitive to cold stress than the wild type (Col-0), although atlrrop2-3 mutant plants were less sensitive to chilling and freezing temperatures than atlrrop2-1 and atlrrop2-2 plants. Taken together, these results suggest that AtLRRop2 plays an important role in cold stress signaling in Arabidopsis .
ISSN:1863-5466
1863-5474
DOI:10.1007/s11816-021-00701-5