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Effects of Lactobacillus curvatus MG5246 on inflammatory markers in Porphyromonas gingivalis lipopolysaccharide-sensitized human gingival fibroblasts and periodontitis rat model
This study investigated the effects of Lactobacillus curvatus MG5246 on periodontitis inflammation. Cell-free supernatants (CFS) prepared from L. curvatus MG5246 decreased prostaglandin E2 production and cyclooxygenase-2 gene expression by 60% and 78% in Porphyromonas gingivalis -lipopolysaccharide...
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| Published in: | Food science and biotechnology 2022, 31(1), , pp.111-120 |
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| cites | cdi_FETCH-LOGICAL-c508t-14fb1edff51fa4dc51ebe8329413e56d34bf63b052eccf7779d3e8c93c9786fb3 |
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| container_title | Food science and biotechnology |
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| creator | Jung, Jae-In Kim, Yong Gyeong Kang, Chang-Ho Imm, Jee-Young |
| description | This study investigated the effects of
Lactobacillus curvatus
MG5246 on periodontitis inflammation. Cell-free supernatants (CFS) prepared from
L. curvatus
MG5246 decreased prostaglandin E2 production and cyclooxygenase-2 gene expression by 60% and 78% in
Porphyromonas gingivalis
-lipopolysaccharide stimulated human gingival fibroblasts at 400 μg/mL. Gene expressions of tumor necrosis factor-α, interleukin-6, matrix metalloproteinases, and chemokines were significantly downregulated by CFS treatment (
p
|
| doi_str_mv | 10.1007/s10068-021-01009-4 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_nrf_k</sourceid><recordid>TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_9916695</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2616980814</sourcerecordid><originalsourceid>FETCH-LOGICAL-c508t-14fb1edff51fa4dc51ebe8329413e56d34bf63b052eccf7779d3e8c93c9786fb3</originalsourceid><addsrcrecordid>eNp9kk2PFCEQhjtG446rf8CDIfGih1Y-Grq5mGw267rJGI1Zz4SmYYYdGlqgJxn_lf9QZmYdPw5eqAr11EtReavqOYJvEITt21RO1tUQoxqWlNfNg2qBIcc1ayh7WC0QxqxuW9idVU9Suis0wm3zuDojFFKOCV1UP66M0SonEAxYSpVDL5V1bk5AzXErc0k-XlPcMBA8sN44OY4yh7gDo4wbHVO5BJ9DnNa7GMbgZQIr61d2K51NwNkpTMHtklRqLaMddJ20Tzbb73oA63mU_oQDY_sYeidTmUb6AUw62jAEnwueQJQZjGHQ7mn1yEiX9LP7eF59fX91e_mhXn66vrm8WNaKwi7XqDE90oMxFBnZDIoi3euOYN4goikbSNMbRnpIsVbKtG3LB6I7xYnibcdMT86r10ddH43YKCuCtIe4CmITxcWX2xvBOWKM08K-O7LT3I96UNrnKJ2Yoi1b2h06_654uy46W9G1hCC8F3h1LxDDt1mnLEablHZOeh3mJDDDGJe5IC_oy3_QuzBHX1ZRKMR4BzvUFAofKRVDSlGb0zAIir17xNE9orhHHNwj9k0v_vzGqeWXXQpAjkAqJb_S8ffb_5H9CYHk1eY</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2616980814</pqid></control><display><type>article</type><title>Effects of Lactobacillus curvatus MG5246 on inflammatory markers in Porphyromonas gingivalis lipopolysaccharide-sensitized human gingival fibroblasts and periodontitis rat model</title><source>PubMed (Medline)</source><source>Springer Link</source><creator>Jung, Jae-In ; Kim, Yong Gyeong ; Kang, Chang-Ho ; Imm, Jee-Young</creator><creatorcontrib>Jung, Jae-In ; Kim, Yong Gyeong ; Kang, Chang-Ho ; Imm, Jee-Young</creatorcontrib><description>This study investigated the effects of
Lactobacillus curvatus
MG5246 on periodontitis inflammation. Cell-free supernatants (CFS) prepared from
L. curvatus
MG5246 decreased prostaglandin E2 production and cyclooxygenase-2 gene expression by 60% and 78% in
Porphyromonas gingivalis
-lipopolysaccharide stimulated human gingival fibroblasts at 400 μg/mL. Gene expressions of tumor necrosis factor-α, interleukin-6, matrix metalloproteinases, and chemokines were significantly downregulated by CFS treatment (
p
< 0.05).
L. curvatus
MG5246 (2 × 10
8
CFU/day, 8 weeks) administration significantly improved alveolar bone loss in the ligature-induced periodontitis rat model. Elevated mRNA expression of the receptor activator of nuclear factor-κB ligand/osteoprotegerin ratio in the gingival tissue was significantly decreased by
L. curvatus
MG5246 administration (
p
< 0.05). Moreover,
L. curvatus
MG5246 showed sufficient tolerance in simulated gastrointestinal conditions (gastric tolerance: 89.48%, intestinal tolerance: 98.62%) and did not show antibiotic resistance and hemolytic activity. Therefore,
L. curvatus
MG5246 has the potential as novel oral probiotics.</description><identifier>ISSN: 1226-7708</identifier><identifier>ISSN: 2092-6456</identifier><identifier>EISSN: 2092-6456</identifier><identifier>DOI: 10.1007/s10068-021-01009-4</identifier><identifier>PMID: 35059235</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Alveolar bone ; Antibiotic resistance ; Antibiotics ; Biomedical materials ; Bone loss ; Chemistry ; Chemistry and Materials Science ; Chemokines ; Cyclooxygenase-2 ; Fibroblasts ; Food Science ; Gene expression ; Gingiva ; Inflammation ; Interleukin 6 ; Lactobacillus curvatus ; Lipopolysaccharides ; Matrix metalloproteinase ; Matrix metalloproteinases ; Nutrition ; Osteoprotegerin ; Periodontitis ; Physical growth ; Porphyromonas gingivalis ; Probiotics ; Prostaglandin E2 ; Research Article ; Tumor necrosis factor-α ; 식품과학</subject><ispartof>Food Science and Biotechnology, 2022, 31(1), , pp.111-120</ispartof><rights>The Korean Society of Food Science and Technology 2021</rights><rights>The Korean Society of Food Science and Technology 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-14fb1edff51fa4dc51ebe8329413e56d34bf63b052eccf7779d3e8c93c9786fb3</citedby><cites>FETCH-LOGICAL-c508t-14fb1edff51fa4dc51ebe8329413e56d34bf63b052eccf7779d3e8c93c9786fb3</cites><orcidid>0000-0002-4715-0669 ; 0000-0001-6466-8550 ; 0000-0003-1970-3315 ; 0000-0003-3152-7051</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733125/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733125/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35059235$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART002802143$$DAccess content in National Research Foundation of Korea (NRF)$$Hfree_for_read</backlink></links><search><creatorcontrib>Jung, Jae-In</creatorcontrib><creatorcontrib>Kim, Yong Gyeong</creatorcontrib><creatorcontrib>Kang, Chang-Ho</creatorcontrib><creatorcontrib>Imm, Jee-Young</creatorcontrib><title>Effects of Lactobacillus curvatus MG5246 on inflammatory markers in Porphyromonas gingivalis lipopolysaccharide-sensitized human gingival fibroblasts and periodontitis rat model</title><title>Food science and biotechnology</title><addtitle>Food Sci Biotechnol</addtitle><addtitle>Food Sci Biotechnol</addtitle><description>This study investigated the effects of
Lactobacillus curvatus
MG5246 on periodontitis inflammation. Cell-free supernatants (CFS) prepared from
L. curvatus
MG5246 decreased prostaglandin E2 production and cyclooxygenase-2 gene expression by 60% and 78% in
Porphyromonas gingivalis
-lipopolysaccharide stimulated human gingival fibroblasts at 400 μg/mL. Gene expressions of tumor necrosis factor-α, interleukin-6, matrix metalloproteinases, and chemokines were significantly downregulated by CFS treatment (
p
< 0.05).
L. curvatus
MG5246 (2 × 10
8
CFU/day, 8 weeks) administration significantly improved alveolar bone loss in the ligature-induced periodontitis rat model. Elevated mRNA expression of the receptor activator of nuclear factor-κB ligand/osteoprotegerin ratio in the gingival tissue was significantly decreased by
L. curvatus
MG5246 administration (
p
< 0.05). Moreover,
L. curvatus
MG5246 showed sufficient tolerance in simulated gastrointestinal conditions (gastric tolerance: 89.48%, intestinal tolerance: 98.62%) and did not show antibiotic resistance and hemolytic activity. Therefore,
L. curvatus
MG5246 has the potential as novel oral probiotics.</description><subject>Alveolar bone</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Biomedical materials</subject><subject>Bone loss</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemokines</subject><subject>Cyclooxygenase-2</subject><subject>Fibroblasts</subject><subject>Food Science</subject><subject>Gene expression</subject><subject>Gingiva</subject><subject>Inflammation</subject><subject>Interleukin 6</subject><subject>Lactobacillus curvatus</subject><subject>Lipopolysaccharides</subject><subject>Matrix metalloproteinase</subject><subject>Matrix metalloproteinases</subject><subject>Nutrition</subject><subject>Osteoprotegerin</subject><subject>Periodontitis</subject><subject>Physical growth</subject><subject>Porphyromonas gingivalis</subject><subject>Probiotics</subject><subject>Prostaglandin E2</subject><subject>Research Article</subject><subject>Tumor necrosis factor-α</subject><subject>식품과학</subject><issn>1226-7708</issn><issn>2092-6456</issn><issn>2092-6456</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kk2PFCEQhjtG446rf8CDIfGih1Y-Grq5mGw267rJGI1Zz4SmYYYdGlqgJxn_lf9QZmYdPw5eqAr11EtReavqOYJvEITt21RO1tUQoxqWlNfNg2qBIcc1ayh7WC0QxqxuW9idVU9Suis0wm3zuDojFFKOCV1UP66M0SonEAxYSpVDL5V1bk5AzXErc0k-XlPcMBA8sN44OY4yh7gDo4wbHVO5BJ9DnNa7GMbgZQIr61d2K51NwNkpTMHtklRqLaMddJ20Tzbb73oA63mU_oQDY_sYeidTmUb6AUw62jAEnwueQJQZjGHQ7mn1yEiX9LP7eF59fX91e_mhXn66vrm8WNaKwi7XqDE90oMxFBnZDIoi3euOYN4goikbSNMbRnpIsVbKtG3LB6I7xYnibcdMT86r10ddH43YKCuCtIe4CmITxcWX2xvBOWKM08K-O7LT3I96UNrnKJ2Yoi1b2h06_654uy46W9G1hCC8F3h1LxDDt1mnLEablHZOeh3mJDDDGJe5IC_oy3_QuzBHX1ZRKMR4BzvUFAofKRVDSlGb0zAIir17xNE9orhHHNwj9k0v_vzGqeWXXQpAjkAqJb_S8ffb_5H9CYHk1eY</recordid><startdate>20220101</startdate><enddate>20220101</enddate><creator>Jung, Jae-In</creator><creator>Kim, Yong Gyeong</creator><creator>Kang, Chang-Ho</creator><creator>Imm, Jee-Young</creator><general>Springer Singapore</general><general>Springer Nature B.V</general><general>한국식품과학회</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>ACYCR</scope><orcidid>https://orcid.org/0000-0002-4715-0669</orcidid><orcidid>https://orcid.org/0000-0001-6466-8550</orcidid><orcidid>https://orcid.org/0000-0003-1970-3315</orcidid><orcidid>https://orcid.org/0000-0003-3152-7051</orcidid></search><sort><creationdate>20220101</creationdate><title>Effects of Lactobacillus curvatus MG5246 on inflammatory markers in Porphyromonas gingivalis lipopolysaccharide-sensitized human gingival fibroblasts and periodontitis rat model</title><author>Jung, Jae-In ; Kim, Yong Gyeong ; Kang, Chang-Ho ; Imm, Jee-Young</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-14fb1edff51fa4dc51ebe8329413e56d34bf63b052eccf7779d3e8c93c9786fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Alveolar bone</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Biomedical materials</topic><topic>Bone loss</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemokines</topic><topic>Cyclooxygenase-2</topic><topic>Fibroblasts</topic><topic>Food Science</topic><topic>Gene expression</topic><topic>Gingiva</topic><topic>Inflammation</topic><topic>Interleukin 6</topic><topic>Lactobacillus curvatus</topic><topic>Lipopolysaccharides</topic><topic>Matrix metalloproteinase</topic><topic>Matrix metalloproteinases</topic><topic>Nutrition</topic><topic>Osteoprotegerin</topic><topic>Periodontitis</topic><topic>Physical growth</topic><topic>Porphyromonas gingivalis</topic><topic>Probiotics</topic><topic>Prostaglandin E2</topic><topic>Research Article</topic><topic>Tumor necrosis factor-α</topic><topic>식품과학</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jung, Jae-In</creatorcontrib><creatorcontrib>Kim, Yong Gyeong</creatorcontrib><creatorcontrib>Kang, Chang-Ho</creatorcontrib><creatorcontrib>Imm, Jee-Young</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Korean Citation Index</collection><jtitle>Food science and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jung, Jae-In</au><au>Kim, Yong Gyeong</au><au>Kang, Chang-Ho</au><au>Imm, Jee-Young</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Lactobacillus curvatus MG5246 on inflammatory markers in Porphyromonas gingivalis lipopolysaccharide-sensitized human gingival fibroblasts and periodontitis rat model</atitle><jtitle>Food science and biotechnology</jtitle><stitle>Food Sci Biotechnol</stitle><addtitle>Food Sci Biotechnol</addtitle><date>2022-01-01</date><risdate>2022</risdate><volume>31</volume><issue>1</issue><spage>111</spage><epage>120</epage><pages>111-120</pages><issn>1226-7708</issn><issn>2092-6456</issn><eissn>2092-6456</eissn><abstract>This study investigated the effects of
Lactobacillus curvatus
MG5246 on periodontitis inflammation. Cell-free supernatants (CFS) prepared from
L. curvatus
MG5246 decreased prostaglandin E2 production and cyclooxygenase-2 gene expression by 60% and 78% in
Porphyromonas gingivalis
-lipopolysaccharide stimulated human gingival fibroblasts at 400 μg/mL. Gene expressions of tumor necrosis factor-α, interleukin-6, matrix metalloproteinases, and chemokines were significantly downregulated by CFS treatment (
p
< 0.05).
L. curvatus
MG5246 (2 × 10
8
CFU/day, 8 weeks) administration significantly improved alveolar bone loss in the ligature-induced periodontitis rat model. Elevated mRNA expression of the receptor activator of nuclear factor-κB ligand/osteoprotegerin ratio in the gingival tissue was significantly decreased by
L. curvatus
MG5246 administration (
p
< 0.05). Moreover,
L. curvatus
MG5246 showed sufficient tolerance in simulated gastrointestinal conditions (gastric tolerance: 89.48%, intestinal tolerance: 98.62%) and did not show antibiotic resistance and hemolytic activity. Therefore,
L. curvatus
MG5246 has the potential as novel oral probiotics.</abstract><cop>Singapore</cop><pub>Springer Singapore</pub><pmid>35059235</pmid><doi>10.1007/s10068-021-01009-4</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-4715-0669</orcidid><orcidid>https://orcid.org/0000-0001-6466-8550</orcidid><orcidid>https://orcid.org/0000-0003-1970-3315</orcidid><orcidid>https://orcid.org/0000-0003-3152-7051</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Food Science and Biotechnology, 2022, 31(1), , pp.111-120 |
| issn | 1226-7708 2092-6456 2092-6456 |
| language | eng |
| recordid | cdi_nrf_kci_oai_kci_go_kr_ARTI_9916695 |
| source | PubMed (Medline); Springer Link |
| subjects | Alveolar bone Antibiotic resistance Antibiotics Biomedical materials Bone loss Chemistry Chemistry and Materials Science Chemokines Cyclooxygenase-2 Fibroblasts Food Science Gene expression Gingiva Inflammation Interleukin 6 Lactobacillus curvatus Lipopolysaccharides Matrix metalloproteinase Matrix metalloproteinases Nutrition Osteoprotegerin Periodontitis Physical growth Porphyromonas gingivalis Probiotics Prostaglandin E2 Research Article Tumor necrosis factor-α 식품과학 |
| title | Effects of Lactobacillus curvatus MG5246 on inflammatory markers in Porphyromonas gingivalis lipopolysaccharide-sensitized human gingival fibroblasts and periodontitis rat model |
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