Evaluation of the Immunological Activity of Gryllus bimaculatus Water Extract

• Published in: Preventive nutrition and food science 2022, 27(1), , pp.99-107
• Main Authors: Lim, Hyun Jung, Han, Jeong Moo, Byun, Eui-Hong
• Format: Article
• Language: English
• Subjects: Original; 식품과학
• ISSN: 2287-1098; 2287-8602
• DOI: 10.3746/pnf.2022.27.1.99

Edible insects are commonly consumed across the world because of their size, availability, and nutritional benefits. They have also been recommended as a potential solution to food shortage because of their high nutritional value. In this study, we demonstrated the immunological effects of on RAW 264.7 cells and splenocytes obtained from mouse. This is the first study to evaluate the immunological effects of water extract. Innate and adaptive immunity were evaluated and measured in RAW 264.7 cells and/or mouse splenocytes using a cell viability assay; changes in cytokine abundance, nitric oxide production, and cell surface molecule abundance were determined using flow cytometry; and western blotting analysis was performed for various immune signaling pathways. water extract showed no cytotoxicity in cells, and the results suggest that treatment with water extract can induce macrophage activation through mitogen-activated protein kinase and nuclear factor-κB signaling, induction of proinflammatory cytokines [interleukin (IL)-6, IL-1β, and tumor necrosis factor-α] and activation of the expression of cell surface molecules [cluster of differentiation (CD)80, CD86, major histocompatibility complex (MHC) class I, and MHC class II]. Treatment with water extract increased the production of cytokines (IL-2, IL-4, and interferon-γ) in splenocytes. The results indicate that water extract can regulate innate and adaptive immunity via modulation macrophages and splenocytes activation and can serve as an immunological agent. We inferred that is a safe and efficient natural material that enhances immunological activity.