Exploiting the antibacterial mechanism of phenazine substances from Lysobacter antibioticus 13-6 against Xanthomonas oryzae pv. oryzicola

• Published in: The journal of microbiology 2022, 60(5), , pp.496-510
• Main Authors: Liu, Qi, Yang, Jun, Ahmed, Waqar, Wan, Xiaoyan, Wei, Lanfang, Ji, Guanghai
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - metabolism; Anti-Bacterial Agents - pharmacology; Antibiotics; Antiinfectives and antibacterials; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biomedical and Life Sciences; Cell membranes; Cell size; Crop production; Cytology; Deformation; Flagella; Gene expression; Genes; Intracellular; Ion transport; Leaf streak; Leaves; Life Sciences; Lysobacter; Membrane permeability; Metabolism; Metabolites; Microbial Pathogenesis and Host-Microbe Interaction; Microbiology; Oryza - microbiology; Pathogenicity; Pathogens; Permeability; Phenazine; Phenazines - metabolism; Phenazines - pharmacology; Phosphates; Physiology; Plant Diseases - microbiology; Plant Diseases - prevention & control; Potassium; Secondary metabolites; Transcriptomics; Transportation systems; Transposition; Xanthomonas; Xanthomonas oryzae; 생물학
• ISSN: 1976-3794; 1225-8873; 1976-3794
• DOI: 10.1007/s12275-022-1542-0

Bacterial leaf streak caused by Xanthomonas oryzae pv. oryzicola ( Xoc ) is one of the most destructive diseases affecting rice production worldwide. In this study, we extracted and purified phenazine substances from the secondary metabolites of Lysobacter antibioticus 13-6. The bacteriostatic mechanism of phenazine substances against Xoc was investigated through physiological response and transcriptomic analysis. Results showed that phenazine substances affects the cell membrane permeability of Xoc , which causes cell swelling and deformation, blockage of flagellum synthesis, and imbalance of intracellular environment. The changes in intracellular environment affect the physiological and metabolic functions of Xoc , which reduces the formation of pathogenic factors and pathogenicity. Through transcriptomic analysis, we found that among differentially expressed genes, the expression of 595 genes was induced significantly (275 up-regulated and 320 down-regulated). In addition, we observed that phenazine substances affects three main functions of Xoc , i.e., transmembrane transporter activity, DNA-mediated transposition, and structural molecular activity. Phenazine substances also inhibits the potassium ion transport system that reduces Xoc resistance and induces the phosphate ion transport system to maintain the stability of the internal environment. Finally, we conclude that phenazine substances could retard cell growth and reduce the pathogenicity of Xoc by affecting cell structure and physiological metabolism. Altogether, our study highlights latest insights into the antibacterial mechanism of phenazine substances against Xoc and provides basic guidance to manage the incidence of bacterial leaf streak of rice.