Lysobacter ciconiae sp. nov., and Lysobacter avium sp. nov., isolated from the faeces of an Oriental stork

• Published in: The journal of microbiology 2022, 60(5), , pp.469-477
• Main Authors: Lee, So-Yeon, Kim, Pil Soo, Sung, Hojun, Hyun, Dong-Wook, Bae, Jin-Woo
• Format: Article
• Language: English
• Subjects: Animals; Aquatic birds; Bacterial Typing Techniques; Biomedical and Life Sciences; Birds - microbiology; Cardiolipin; Coenzyme Q8; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Faeces; Fatty acids; Fatty Acids - analysis; Feces; Feces - microbiology; Gene sequencing; Genes; Genomes; Life Sciences; Lipids; Lysobacter; Microbial Systematics and Evolutionary Microbiology; Microbiological strains; Microbiology; New species; Nucleotides; pH effects; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids - analysis; Phylogeny; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sodium Chloride; Strains (organisms); Waterfowl; Whole genome sequencing; 생물학
• ISSN: 1976-3794; 1225-8873; 1976-3794
• DOI: 10.1007/s12275-022-1647-5

Two Gram-stain-negative, mesophilic, strictly aerobic, nonspore forming, and yellow-pigmented strains with rod-shaped cells, designated H21R20 T and H23M41 T , were isolated from the faeces of an Oriental stork ( Ciconia boyciana ). Based on 16S rRNA gene sequences, both strains showed the highest similarity (98.3–98.4%) to the type strain of Lysobacter concretionis. Phylogenetic analysis based on the 16S rRNA genes and 92 bacterial core genes showed that strains H21R20 T and H23M41 T were robustly clustered with L. concretionis Ko07 T . Whole genome sequencing revealed that the genomes of both strains were approximately 2.9 Mb in size. The DNA G + C contents of the H21R20 T and H23M41 T strains were 67.3 and 66.6%, respectively. The two strains showed 80.1–81.7% average nucleotide identity with L. concretionis Ko07 T . Strain H21R20 T grew optimally at 30°C and pH 8.0 and in the presence of 0.5–3% (wt/vol) NaCl, while strain H23M41 T grew optimally at 30°C and pH 7.0–8.0 and in the presence of 0–3% (wt/vol) NaCl. Both strains possessed iso-C 15:0 , iso-C 16:0 and summed feature 9 (iso-C 17:1 ω 9c and/or C 16:0 10-methyl) as the major cellular fatty acids, ubiquinone Q-8 as a predominant quinone, and diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as the major polar lipids. A multifaceted investigation demonstrated that strains H21R20 T and H23M41 T represent novel species of the genus Lysobacter , for which we propose the names Lysobacter ciconiae sp. nov. and Lysobacter avium sp. nov. for strains H21R20 T (= KCTC 82316 T = JCM 34832 T ) and H23M41 T (= KCTC 62676 T = JCM 33223 T ), respectively.