Assessment of Populus wood chemistry following the introduction of a Bt toxin gene

Unintended changes in plant physiology, anatomy and metabolism as a result of genetic engineering are a concern as more transgenic plants are commercially deployed in the ecosystem. We compared the cell wall chemical composition of three Populus lines (Populus trichocarpa Torr. & A. Gray x Popul...

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Bibliographic Details
Published in:Tree physiology 2006-05, Vol.26 (5), p.557-564
Main Authors: Davis, M.F, Tuskan, G.A, Payne, P, Tschaplinski, T.J, Meilan, R
Format: Article
Language:English
Subjects:
09 BIOMASS FUELS
Agrobacterium tumefaciens
ANATOMY
BACILLUS
Bacillus thuringiensis
Bacillus thuringiensis - genetics
bacterial toxins
Bacterial Toxins - genetics
CELL WALL
Cell Wall - metabolism
cell wall components
CHEMICAL COMPOSITION
CHEMISTRY
dry matter accumulation
forest trees
Gas Chromatography-Mass Spectrometry
gene expression
gene transfer
GENES
GENETIC ENGINEERING
genetic transformation
Genetic Vectors
Mass Spectrometry
MASS SPECTROSCOPY
METABOLISM
MOLECULAR BEAMS
PHYSIOLOGY
Plants, Genetically Modified
Populus - genetics
Populus - metabolism
Populus balsamifera subsp. trichocarpa
Populus deltoides
Populus nigra
PROTEINS
PYROLYSIS
quantitative analysis
SPECTROSCOPY
TOXINS
TRANSGENIC PLANTS
WOOD
Wood - chemistry
Wood - metabolism
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Summary:Unintended changes in plant physiology, anatomy and metabolism as a result of genetic engineering are a concern as more transgenic plants are commercially deployed in the ecosystem. We compared the cell wall chemical composition of three Populus lines (Populus trichocarpa Torr. & A. Gray x Populus deltoides Bartr. ex Marsh., Populus trichocarpa x Populus nigra L. and Populus deltoides x Populus nigra) genetically modified to express the Cry3A or Cry3B2 protein of Bacillus thuringiensis (Bt) with the cell wall chemistry of non-transformed isogenic control lines. Three genetically modified clones, each represented by 10 independent transgenic lines, were analyzed by pyrolysis molecular beam mass spectrometry, gas chromatography/mass spectrometry and traditional wet chemical analytical methods to assess changes in cell wall composition. Based on the outcome of these techniques, there were no comprehensive differences in chemical composition between the transgenic and control lines for any of the studied clones.
ISSN:0829-318X
1758-4469
DOI:10.1093/treephys/26.5.557