Fluorescence of bioaerosols: mathematical model including primary fluorescing and absorbing molecules in bacteria

• Published in: Optics express 2013-09, Vol.21 (19), p.22285-22313
• Main Authors: Hill, Steven C, Pan, Yong-Le, Williamson, Chatt, Santarpia, Joshua L, Hill, Hanna H
• Format: Article
• Language: English
• Subjects: Absorption; Aerosols - chemistry; Aerosols - metabolism; Bacteria - chemistry; Bacteria - metabolism; Biopolymers - chemistry; Computer Simulation; Fluorescence; Light; Models, Biological; Models, Chemical; Spectrometry, Fluorescence - methods
• ISSN: 1094-4087; 1094-4087
• DOI: 10.1364/OE.21.022285

This paper describes a mathematical model of fluorescent biological particles composed of bacteria, viruses, or proteins. The fluorescent and/or light absorbing molecules included in the model are amino acids (tryptophan, etc.); nucleic acids (DNA, RNA, etc.); coenzymes (nicotinamide adenine dinucleotides, flavins, and vitamins B₆ and K and variants of these); and dipicolinates. The concentrations, absorptivities, and fluorescence quantum yields are estimated from the literature, often with large uncertainties. The bioparticles in the model are spherical and homogeneous. Calculated fluorescence cross sections for particles excited at 266, 280, and 355 nm are compared with measured values from the literature for several bacteria, bacterial spores and albumins. The calculated 266- and 280-nm excited fluorescence is within a factor of 3.2 of the measurements for the vegetative cells and proteins, but overestimates the fluorescence of spores by a factor of 10 or more. This is the first reported modeling of the fluorescence of bioaerosols in which the primary fluorophores and absorbing molecules are included.