Zn(II) Binding to Escherichia coli 70S Ribosomes

Escherichia coli 70S ribosomes tightly bind 8 equiv of Zn(II), and EXAFS spectra indicate that Zn(II) may be protein-bound. Ribosomes were incubated with EDTA and Zn(II), and after dialysis, the resulting ribosomes bound 5 and 11 equiv of Zn(II), respectively. EXAFS studies show that the additional...

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Bibliographic Details
Published in:Biochemistry (Easton) 2011-11, Vol.50 (46), p.9937-9939
Main Authors: Hensley, M. Patrick, Tierney, David L, Crowder, Michael W
Format: Article
Language:English
Subjects:
Binding Sites
Escherichia coli
Escherichia coli - metabolism
Escherichia coli Proteins - metabolism
Metalloproteins - metabolism
Models, Molecular
Phosphates - metabolism
Ribosomal Proteins - metabolism
Ribosome Subunits, Large, Bacterial - metabolism
Ribosome Subunits, Small, Bacterial - metabolism
Zinc - metabolism
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Summary:Escherichia coli 70S ribosomes tightly bind 8 equiv of Zn(II), and EXAFS spectra indicate that Zn(II) may be protein-bound. Ribosomes were incubated with EDTA and Zn(II), and after dialysis, the resulting ribosomes bound 5 and 11 equiv of Zn(II), respectively. EXAFS studies show that the additional Zn(II) in the zinc-supplemented ribosomes binds in part to the phosphate backbone of the ribosome. Lastly, in vitro translation studies demonstrate that EDTA-treated ribosomes do not synthesize an active Zn(II)-bound metalloenzyme, while the as-isolated ribosomes do. These studies demonstrate that the majority of intracellular Zn(II) resides in the ribosome.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi200619w