Multiple microscopic approaches demonstrate linkage between chromoplast architecture and carotenoid composition in diverse Capsicum annuum fruit

Summary Increased accumulation of specific carotenoids in plastids through plant breeding or genetic engineering requires an understanding of the limitations that storage sites for these compounds may impose on that accumulation. Here, using Capsicum annuum L. fruit, we demonstrate directly the uniq...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 2013-12, Vol.76 (6), p.1074-1083
Main Authors: Kilcrease, James, Collins, Aaron M., Richins, Richard D., Timlin, Jerilyn A., O'Connell, Mary A.
Format: Article
Language:English
Subjects:
Capsicum - metabolism
Capsicum - ultrastructure
Capsicum annuum
carotenoids
Carotenoids - analysis
Carotenoids - metabolism
chromoplast
electron microscopy
Fruit - metabolism
Fruit - ultrastructure
Fruits
hyperspectral confocal Raman microscopy
Microscopy
Microscopy, Confocal
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Multivariate Analysis
pericarp
Phenotype
Pigments
Plant biology
Plastids
Plastids - metabolism
Plastids - ultrastructure
SOLAR ENERGY
Species Specificity
technical advance
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Summary:Summary Increased accumulation of specific carotenoids in plastids through plant breeding or genetic engineering requires an understanding of the limitations that storage sites for these compounds may impose on that accumulation. Here, using Capsicum annuum L. fruit, we demonstrate directly the unique sub‐organellar accumulation sites of specific carotenoids using live cell hyperspectral confocal Raman microscopy. Further, we show that chromoplasts from specific cultivars vary in shape and size, and these structural variations are associated with carotenoid compositional differences. Live‐cell imaging utilizing laser scanning confocal (LSCM) and confocal Raman microscopy, as well as fixed tissue imaging by scanning and transmission electron microscopy (SEM and TEM), all demonstrated morphological differences with high concordance for the measurements across the multiple imaging modalities. These results reveal additional opportunities for genetic controls on fruit color and carotenoid‐based phenotypes.
ISSN:0960-7412
1365-313X
DOI:10.1111/tpj.12351