IMAGINE: first neutron protein structure and new capabilities for neutron macromolecular crystallography

The first high-resolution neutron protein structure of perdeuterated rubredoxin from Pyrococcus furiosus (PfRd) determined using the new IMAGINE macromolecular neutron crystallography instrument at the Oak Ridge National Laboratory is reported. Neutron diffraction data extending to 1.65 Å resolution...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2013-09, Vol.69 (10)
Main Authors: Meilleur, Flora, Munshi, Parthapratim, Robertson, Lee, Stoica, Alexandru D., Crow, Lowell, Kovalevsky, Andrey, Koritsanszky, Tibor, Chakoumakos, Bryan C., Blessing, Robert, Myles, Dean A. A.
Format: Article
Language:English
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Summary:The first high-resolution neutron protein structure of perdeuterated rubredoxin from Pyrococcus furiosus (PfRd) determined using the new IMAGINE macromolecular neutron crystallography instrument at the Oak Ridge National Laboratory is reported. Neutron diffraction data extending to 1.65 Å resolution were collected from a relatively small 0.7 mm3 PfRd crystal using 2.5 d (60 h) of beam time. The refined structure contains 371 out of 391, or 95%, of the D atoms of the protein and 58 solvent molecules. The IMAGINE instrument is designed to provide neutron data at or near atomic resolution (1.5 Å) from crystals with volume
ISSN:0907-4449
1399-0047
DOI:10.1107/S0907444913019604