Engineering of a Tyrosol-Producing Pathway, Utilizing Simple Sugar and the Central Metabolic Tyrosine, in Escherichia coli

Metabolic engineering was applied to the development of Escherichia coli capable of synthesizing tyrosol (2-(4-hydroxyphenyl)ethanol), an attractive phenolic compound with great industrial value, from glucose, a renewable carbon source. In this strain, tyrosine, which was supplied not only from the...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2012-02, Vol.60 (4), p.979-984
Main Authors: Satoh, Yasuharu, Tajima, Kenji, Munekata, Masanobu, Keasling, Jay D, Lee, Taek Soon
Format: Article
Language:English
Subjects:
Aldehyde Oxidoreductases - genetics
Aldehyde Oxidoreductases - metabolism
Biological and medical sciences
carbon
culture media
decarboxylation
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli Proteins
ethanol
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
genes
glucose
Glucose - metabolism
Metabolic Engineering
metabolism
mutants
oxidation
phenylacetaldehyde
Phenylacetates - metabolism
Phenylethyl Alcohol - analogs & derivatives
Phenylethyl Alcohol - metabolism
tyrosine
Tyrosine - metabolism
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Summary:Metabolic engineering was applied to the development of Escherichia coli capable of synthesizing tyrosol (2-(4-hydroxyphenyl)ethanol), an attractive phenolic compound with great industrial value, from glucose, a renewable carbon source. In this strain, tyrosine, which was supplied not only from the culture medium but also from the central metabolism, was converted into tyrosol via three steps: decarboxylation, amine oxidation, and reduction. The engineered strain synthesized both tyrosol and 4-hydroxyphenylacetate (4HPA), but disruption of the endogenous phenylacetaldehyde dehydrogenase gene shut off 4HPA production and improved the production of tyrosol as a sole product. The engineered mutant strain was capable of producing 0.5 mM tyrosol from 1% (w/v) glucose during a 48 h shake flask cultivation.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf203256f