Iron nutrition and physiological responses to iron stress in Nitrosomonas europaea

Nitrosomonas europaea, as an ammonia-oxidizing bacterium, has a high Fe requirement and has 90 genes dedicated to Fe acquisition. Under Fe-limiting conditions (0.2 microM Fe), N. europaea was able to assimilate up to 70% of the available Fe in the medium even though it is unable to produce sideropho...

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Bibliographic Details
Published in:Archives of microbiology 2006-08, Vol.186 (2), p.107-118
Main Authors: XUEMING WEI, VAJRALA, Neeraja, HAUSER, Loren, SAYAVEDRA-SOTO, Luis A, ARP, Daniel J
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Bacteria
Bacterial Outer Membrane Proteins - biosynthesis
Bacterial Outer Membrane Proteins - isolation & purification
Bacterial Proteins - biosynthesis
Bacterial Proteins - isolation & purification
Bacteriology
Biological and medical sciences
Biomass
Cell Membrane - ultrastructure
Cytoplasm - chemistry
Fundamental and applied biological sciences. Psychology
Heme - analysis
Iron - metabolism
Mass Spectrometry
Microbiology
Microscopy, Electron, Transmission
Miscellaneous
Nitrosomonas europaea
Nitrosomonas europaea - chemistry
Nitrosomonas europaea - growth & development
Nitrosomonas europaea - metabolism
Nitrosomonas europaea - physiology
Oxygen Consumption
Physiological responses
Porins - biosynthesis
Porins - isolation & purification
Receptors, Cell Surface - biosynthesis
Receptors, Cell Surface - isolation & purification
Siderophores - metabolism
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Summary:Nitrosomonas europaea, as an ammonia-oxidizing bacterium, has a high Fe requirement and has 90 genes dedicated to Fe acquisition. Under Fe-limiting conditions (0.2 microM Fe), N. europaea was able to assimilate up to 70% of the available Fe in the medium even though it is unable to produce siderophores. Addition of exogenous siderophores to Fe-limited medium increased growth (final cell mass). Fe-limited cells had lower heme and cellular Fe contents, reduced membrane layers, and lower NH3- and NH2OH-dependent O2 consumption activities than Fe-replete cells. Fe acquisition-related proteins, such as a number of TonB-dependent Fe-siderophore receptors for ferrichrome and enterobactin and diffusion protein OmpC, were expressed to higher levels under Fe limitation, providing biochemical evidence for adaptation of N. europaea to Fe-limited conditions.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-006-0126-4