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Polyhydroxybutyrate particles in Synechocystis sp. PCC 6803: facts and fiction
Transmission electron microscopy has been used to identify poly-3-hydroxybutyrate (PHB) granules in cyanobacteria for over 40 years. Spherical inclusions inside the cell that are electron-transparent and/or slightly electron-dense and that are found in transmission electron micrographs of cyanobacte...
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| Published in: | Photosynthesis research 2013-11, Vol.118 (1-2), p.37-49 |
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| container_end_page | 49 |
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| creator | Tsang, Tin Ki Roberson, Robert W Vermaas, Wim F. J |
| description | Transmission electron microscopy has been used to identify poly-3-hydroxybutyrate (PHB) granules in cyanobacteria for over 40 years. Spherical inclusions inside the cell that are electron-transparent and/or slightly electron-dense and that are found in transmission electron micrographs of cyanobacteria are generally assumed to be PHB granules. The aim of this study was to test this assumption in different strains of the cyanobacterium Synechocystis sp. PCC 6803. Inclusions that resemble PHB granules were present in strains lacking a pair of genes essential for PHB synthesis and in wild-type cells under conditions that no PHB granules could be detected by fluorescence staining of PHB. Indeed, in these cells PHB could not be demonstrated chemically by GC/MS either. Based on the results gathered, it is concluded that not all the slightly electron-dense spherical inclusions are PHB granules in Synechocystis sp. PCC 6803. This result is potentially applicable to other cyanobacteria. Alternate assignments for these inclusions are discussed. |
| doi_str_mv | 10.1007/s11120-013-9923-1 |
| format | article |
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Indeed, in these cells PHB could not be demonstrated chemically by GC/MS either. Based on the results gathered, it is concluded that not all the slightly electron-dense spherical inclusions are PHB granules in Synechocystis sp. PCC 6803. This result is potentially applicable to other cyanobacteria. 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J</creatorcontrib><title>Polyhydroxybutyrate particles in Synechocystis sp. PCC 6803: facts and fiction</title><title>Photosynthesis research</title><addtitle>Photosynth Res</addtitle><addtitle>Photosynth Res</addtitle><description>Transmission electron microscopy has been used to identify poly-3-hydroxybutyrate (PHB) granules in cyanobacteria for over 40 years. Spherical inclusions inside the cell that are electron-transparent and/or slightly electron-dense and that are found in transmission electron micrographs of cyanobacteria are generally assumed to be PHB granules. The aim of this study was to test this assumption in different strains of the cyanobacterium Synechocystis sp. PCC 6803. Inclusions that resemble PHB granules were present in strains lacking a pair of genes essential for PHB synthesis and in wild-type cells under conditions that no PHB granules could be detected by fluorescence staining of PHB. 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Alternate assignments for these inclusions are discussed.</description><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biopolymers</subject><subject>Cells</subject><subject>Cyanobacteria</subject><subject>Fluorescence</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>genes</subject><subject>granules</subject><subject>Hydroxybutyrates - administration & dosage</subject><subject>Hydroxybutyrates - analysis</subject><subject>Hydroxybutyrates - metabolism</subject><subject>Life Sciences</subject><subject>Microscopy, Electron, Transmission</subject><subject>Microscopy, Fluorescence</subject><subject>Photosynthesis</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polyesters - administration & dosage</subject><subject>Polyesters - analysis</subject><subject>Polyesters - metabolism</subject><subject>polyhydroxybutyrate</subject><subject>Polymers</subject><subject>Regular Paper</subject><subject>Synechocystis</subject><subject>Synechocystis - chemistry</subject><subject>Synechocystis - metabolism</subject><subject>Synechocystis - ultrastructure</subject><subject>Transmission electron microscopy</subject><issn>0166-8595</issn><issn>1573-5079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqNkktv1DAUhS0EosPAD2ADEWxgkcGP-MWuGrVQqYKKoWvLsZ2pq0w82I7U_HscpSDKAiEvLNnfuffcqwPASwQ3CEL-ISGEMKwhIrWUmNToEVghyklNIZePwQoixmpBJT0Bz1K6hRAKhshTcIIbSDFmcgW-XIV-uplsDHdTO-Yp6uyqo47Zm96lyg_VbhqcuQlmStmnKh031dV2WzEByceq0yanSg-26rzJPgzPwZNO98m9uL_X4Pr87Pv2c3359dPF9vSyNsVNrhtBnZFYMKgNJrhpiGhbQx3viDQIdcRQizmzggnZtlwIaQ23lllrHGUdJ2vwZqkbiiuVjM_FowlDsZoVwgjO-1iDdwt0jOHH6FJWB5-M63s9uDAmhRomCOMSw_9AqUACk7K-NXj7F3obxjiUaQvVlL4cwrn3ZqH2unfKD13IUZtyrDv4YtR1vryfEopFIzmTRfD-gaAw2d3lvR5TUhe7bw9ZtLAmhpSi69Qx-oOOk0JQzclQSzJUSYaaN6Fm26_ubY_twdnfil9RKABegFS-hr2Lf8z1j6qvF1Gng9L76JO63mGImjlrWLKG_AT_wshw</recordid><startdate>20131101</startdate><enddate>20131101</enddate><creator>Tsang, Tin Ki</creator><creator>Roberson, Robert W</creator><creator>Vermaas, Wim F. 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| ispartof | Photosynthesis research, 2013-11, Vol.118 (1-2), p.37-49 |
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| subjects | Bacteria Biochemistry Biomedical and Life Sciences Biopolymers Cells Cyanobacteria Fluorescence Gas Chromatography-Mass Spectrometry genes granules Hydroxybutyrates - administration & dosage Hydroxybutyrates - analysis Hydroxybutyrates - metabolism Life Sciences Microscopy, Electron, Transmission Microscopy, Fluorescence Photosynthesis Plant Genetics and Genomics Plant Physiology Plant Sciences Polyesters - administration & dosage Polyesters - analysis Polyesters - metabolism polyhydroxybutyrate Polymers Regular Paper Synechocystis Synechocystis - chemistry Synechocystis - metabolism Synechocystis - ultrastructure Transmission electron microscopy |
| title | Polyhydroxybutyrate particles in Synechocystis sp. PCC 6803: facts and fiction |
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