A Metal Organic Framework with Spherical Protein Nodes: Rational Chemical Design of 3D Protein Crystals

We describe here the construction of a three-dimensional, porous, crystalline framework formed by spherical protein nodes that assemble into a prescribed lattice arrangement through metal–organic linker-directed interactions. The octahedral iron storage enzyme, ferritin, was engineered in its C 3 sy...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the American Chemical Society 2015-09, Vol.137 (36), p.11598-11601
Main Authors: Sontz, Pamela A, Bailey, Jake B, Ahn, Sunhyung, Tezcan, F. Akif
Format: Article
Language:English
Subjects:
Crystallization
Dynamic Light Scattering
Metals - chemistry
Protein Conformation
Proteins - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We describe here the construction of a three-dimensional, porous, crystalline framework formed by spherical protein nodes that assemble into a prescribed lattice arrangement through metal–organic linker-directed interactions. The octahedral iron storage enzyme, ferritin, was engineered in its C 3 symmetric pores with tripodal Zn coordination sites. Dynamic light scattering and crystallographic studies established that this Zn-ferritin construct could robustly self-assemble into the desired bcc-type crystals upon coordination of a ditopic linker bearing hydroxamic acid functional groups. This system represents the first example of a ternary protein–metal–organic crystalline framework whose formation is fully dependent on each of its three components.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.5b07463