Efficiency of light harvesting in a photosynthetic bacterium adapted to different levels of light

• Published in: Biochimica et biophysica acta 2014-10, Vol.1837 (10), p.1835-1846
• Main Authors: Timpmann, Kõu, Chenchiliyan, Manoop, Jalviste, Erko, Timney, John A., Hunter, C. Neil, Freiberg, Arvi
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological; BASIC BIOLOGICAL SCIENCES; Exciton; Fluorescence; Kinetics; Light; Light harvesting; Light-Harvesting Protein Complexes - physiology; Optical spectroscopy; Photosynthesis; Photosynthetic unit; Picosecond excitation energy transfer; Rhodobacter sphaeroides - metabolism; Rhodobacter sphaeroides - physiology; SOLAR ENERGY
• ISSN: 0005-2728; 0006-3002; 1879-2650
• DOI: 10.1016/j.bbabio.2014.06.007

In this study, we use the photosynthetic purple bacterium Rhodobacter sphaeroides to find out how the acclimation of photosynthetic apparatus to growth conditions influences the rates of energy migration toward the reaction center traps and the efficiency of charge separation at the reaction centers. To answer these questions we measured the spectral and picosecond kinetic fluorescence responses as a function of excitation intensity in membranes prepared from cells grown under different illumination conditions. A kinetic model analysis yielded the microscopic rate constants that characterize the energy transfer and trapping inside the photosynthetic unit as well as the dependence of exciton trapping efficiency on the ratio of the peripheral LH2 and core LH1 antenna complexes, and on the wavelength of the excitation light. A high quantum efficiency of trapping over 80% was observed in most cases, which decreased toward shorter excitation wavelengths within the near infrared absorption band. At a fixed excitation wavelength the efficiency declines with the LH2/LH1 ratio. From the perspective of the ecological habitat of the bacteria the higher population of peripheral antenna facilitates growth under dim light even though the energy trapping is slower in low light adapted membranes. The similar values for the trapping efficiencies in all samples imply a robust photosynthetic apparatus that functions effectively at a variety of light intensities. [Display omitted] •In photosynthetic bacteria the rates of energy migration depend on growth conditions.•Adaptation to low light slows down the migration rate, retaining the high energy trapping efficiency.•The quantum efficiency decreases toward shorter excitation wavelengths within the Qy absorption band.•Neglecting losses due to LH2, the exciton trapping time is ~61ps irrespective of the sample.•The high light samples contain a small population of disconnected peripheral antenna complexes.