Biosensor for label-free DNA quantification based on functionalized LPGs

A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonan...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2016-10, Vol.84 (C), p.30-36
Main Authors: Gonçalves, Helena M.R., Moreira, Luis, Pereira, Leonor, Jorge, Pedro, Gouveia, Carlos, Martins-Lopes, Paula, Fernandes, José R.A.
Format: Article
Language:English
Subjects:
Base Pair Mismatch
Biosensing
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Deoxyribonucleic acid
Detection
Diffraction gratings
DNA
DNA - analysis
DNA - genetics
Equipment Design
Fiber Optic Technology - instrumentation
Fiber Optic Technology - methods
In Situ Hybridization - instrumentation
In Situ Hybridization - methods
Interrogation
Label-free
Limit of Detection
LPG
Models, Molecular
Optical fibers
Quantification
Spectra
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Summary:A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62±2nM and the limit of quantification was 209±7nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity. •DNA hybridization sensing.•Optical fiber sensor.•Simple optical interrogation scheme.•DNA sequence detection.•DNA quantification.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2015.10.001