Quantum Dot Nano Thermometers Reveal Heterogeneous Local Thermogenesis in Living Cells

The local temperature response inside single living cells upon external chemical and physical stimuli was characterized using quantum dots as nano thermometers. The photoluminescence spectral shifts from endocytosed quantum dots were used to map intracellular heat generation in NIH/3T3 cells followi...

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Bibliographic Details
Published in:ACS nano 2011-06, Vol.5 (6), p.5067-5071
Main Authors: Yang, Jui-Ming, Yang, Haw, Lin, Liwei
Format: Article
Language:English
Subjects:
Animals
BASIC BIOLOGICAL SCIENCES
Biotechnology - methods
Calcium - chemistry
intracellular temperature
Mice
nanobio
NANOSCIENCE AND NANOTECHNOLOGY
Nanostructures
Nanotechnology - methods
NIH 3T3 Cells
Photons
Quantum Dots
single-cell analysis
spectral imaging
Temperature
thermal signaling
Thermometers
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Summary:The local temperature response inside single living cells upon external chemical and physical stimuli was characterized using quantum dots as nano thermometers. The photoluminescence spectral shifts from endocytosed quantum dots were used to map intracellular heat generation in NIH/3T3 cells following Ca2+ stress and cold shock. The direct observation of inhomogeneous intracellular temperature progression raises interesting new possibilities, including further innovations in nanomaterials for sensing local responses, as well as the concept of subcellular temperature gradient for signaling and regulation in cells.
ISSN:1936-0851
1936-086X
DOI:10.1021/nn201142f