Assessing compartmentalized flux in lipid metabolism with isotopes

• Published in: Biochimica et biophysica acta 2016-09, Vol.1861 (9), p.1226-1242
• Main Author: Allen, Doug K.
• Format: Article
• Language: English
• Subjects: 07 ISOTOPE AND RADIATION SOURCES; Acetyl Coenzyme A - chemistry; Acetyl Coenzyme A - metabolism; BASIC BIOLOGICAL SCIENCES; Carbon Isotopes - chemistry; Cell Compartmentation - genetics; Cellular heterogeneity; Central metabolism; Isotopic labeling; Lipid metabolism; Lipid Metabolism - genetics; Metabolic Flux Analysis; Plants - metabolism; Plastids - metabolism; Subcellular compartmentation
• ISSN: 1388-1981; 0006-3002; 1879-2618
• DOI: 10.1016/j.bbalip.2016.03.017

Metabolism in plants takes place across multiple cell types and within distinct organelles. The distributions equate to spatial heterogeneity; though the limited means to experimentally assess metabolism frequently involve homogenizing tissues and mixing metabolites from different locations. Most current isotope investigations of metabolism therefore lack the ability to resolve spatially distinct events. Recognition of this limitation has resulted in inspired efforts to advance metabolic flux analysis and isotopic labeling techniques. Though a number of these efforts have been applied to studies in central metabolism; recent advances in instrumentation and techniques present an untapped opportunity to make similar progress in lipid metabolism where the use of stable isotopes has been more limited. These efforts will benefit from sophisticated radiolabeling reports that continue to enrich our knowledge on lipid biosynthetic pathways and provide some direction for stable isotope experimental design and extension of MFA. Evidence for this assertion is presented through the review of several elegant stable isotope studies and by taking stock of what has been learned from radioisotope investigations when spatial aspects of metabolism were considered. The studies emphasize that glycerolipid production occurs across several locations with assembly of lipids in the ER or plastid, fatty acid biosynthesis occurring in the plastid, and the generation of acetyl-CoA and glycerol-3-phosphate taking place at multiple sites. Considering metabolism in this context underscores the cellular and subcellular organization that is important to enhanced production of glycerolipids in plants. An attempt is made to unify salient features from a number of reports into a diagrammatic model of lipid metabolism and propose where stable isotope labeling experiments and further flux analysis may help address questions in the field. This article is part of a Special Issue entitled: Plant Lipid Biology edited by Kent D. Chapman and Ivo Feussner. •Isotopic labeling has provided numerous insights to lipid and central metabolism.•Cellular heterogeneity and subcellular compartmentation complicate experiments.•Stable isotopes with mass spectrometry can address current questions.•Recent advances in metabolic flux analysis will benefit studies in lipid metabolism.