The NADPH-oxidase AtRbohI plays a positive role in drought-stress response in Arabidopsis thaliana

• Published in: Biochemical and biophysical research communications 2017-09, Vol.491 (3), p.834-839
• Main Authors: He, Huan, Yan, Jingwei, Yu, Xiaoyun, Liang, Yan, Fang, Lin, Scheller, Henrik Vibe, Zhang, Aying
• Format: Article
• Language: English
• Subjects: Arabidopsis - physiology; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; AtRbohI; BASIC BIOLOGICAL SCIENCES; Drought stress; Droughts; Gene Expression Regulation, Enzymologic - physiology; Gene Expression Regulation, Plant - physiology; Heat-Shock Response - physiology; INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; NADPH Oxidases - metabolism; Reactive oxygen species
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2017.05.131

As the major resource of reactive oxygen species (ROS), the NADPH oxidases (Rbohs) have been shown to play important roles in plant cells under normal growth and stress conditions. Although many family members of Rbohs were studied, little is known about the function of RbohI in Arabidopsis thaliana. Here, we report that exogenous ABA application decreases RbohI expression and mannitol significantly increases RbohI expression at transcript level. The RbohI transcripts were strongly detected in dry seeds and roots. The loss-of-function mutant rbohI exhibited sensitivity to ABA and mannitol stress during germination. Furthermore, the lateral root growth of rbohI was severely inhibited after treatment with mannitol stress. Overexpression of RbohI in Arabidopsis significantly improves the drought tolerance. Moreover, more H2O2 accumulated in RbohI overexpressors than in wild type plants in response to mannitol stress. Our conclusion is that AtRbohI functions in drought-stress response in Arabidopsis thaliana. •Manntiol stress could significantly increase RbohI expression.•RbohI Mutant is sensitive to osmotic stress in root.•Overexpression of RbohI in Arabidopsis obviously elevated drought tolerance.