Flow-Through Porous Silicon Membranes for Real-Time Label-Free Biosensing

A flow-through sensing platform based on open-ended porous silicon (PSi) microcavity membranes that are compatible with integration in on-chip sensor arrays is demonstrated. Because of the high aspect ratio of PSi nanopores, the performance of closed-ended PSi sensors is limited by infiltration chal...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2016-11, Vol.88 (22), p.10940-10948
Main Authors: Zhao, Yiliang, Gaur, Girija, Retterer, Scott T, Laibinis, Paul E, Weiss, Sharon M
Format: Article
Language:English
Subjects:
Binding sites
Detection
INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
MATERIALS SCIENCE
Membranes
Microcavities
Molecular weight
Molecules
Multiplexing
Porosity
Porous silicon
Proteins
Response time
Sensors
Silicon
Simulation
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Summary:A flow-through sensing platform based on open-ended porous silicon (PSi) microcavity membranes that are compatible with integration in on-chip sensor arrays is demonstrated. Because of the high aspect ratio of PSi nanopores, the performance of closed-ended PSi sensors is limited by infiltration challenges and slow sensor responses when detecting large molecules such as proteins and nucleic acids. In order to improve molecule transport efficiency and reduce sensor response time, open-ended PSi nanopore membranes were used in a flow-through sensing scheme, allowing analyte solutions to pass through the nanopores. The molecular binding kinetics in these PSi membranes were compared through experiments and simulation with those from closed-ended PSi films of comparable thickness in a conventional flow-over sensing scheme. The flow-through PSi membrane resulted in a 6-fold improvement in sensor response time when detecting a high molecular weight analyte (streptavidin) versus in the flow-over PSi approach. This work demonstrates the possibility of integrating multiple flow-through PSi sensor membranes within parallel microarrays for rapid and multiplexed label-free biosensing.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.6b02521