Evaluation of the performance of the IQ‐Check kits and the USDA Microbiology Laboratory Guidebook methods for detection of Shiga toxin‐producing Escherichia coli (STEC) and STEC and Salmonella simultaneously in ground beef

• Published in: Journal of applied microbiology 2017-03, Vol.122 (3), p.809-816
• Main Authors: Baranzoni, G.M., Fratamico, P.M., Boccia, F., Bagi, L.K., Kim, G.‐H., Anastasio, A., Pepe, T.
• Format: Article
• Language: English
• Subjects: Animals; Cattle; detection; E coli; Escherichia coli; Food contamination & poisoning; Food Microbiology; food safety; Guidelines as Topic; meat; Microbiology; PCR; Polymerase Chain Reaction - methods; Polymerase Chain Reaction - standards; Red Meat - microbiology; Salmonella; Salmonella - genetics; Salmonella - isolation & purification; Salmonella typhimurium; Shiga Toxins - analysis; Shiga Toxins - genetics; Shiga-Toxigenic Escherichia coli - genetics; Shiga-Toxigenic Escherichia coli - isolation & purification; STEC; United States; United States Department of Agriculture; Virulence Factors - genetics
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.13380

Aims To evaluate the performance of the IQ‐Check kits and the USDA Microbiology Laboratory Guidebook (MLG) methods for detection of the top seven Shiga toxin‐producing Escherichia coli (STEC) (O157:H7, O26, O45, O103, O111, O121 and O145) in ground beef and both STEC and Salmonella in co‐inoculated samples. Methods and Results Ground beef samples inoculated with ~10 CFU of STEC or both STEC and Salmonella Typhimurium were stored at 4°C for 72 h, followed by screening with the IQ‐Check and BAX System kit (MLG) methods that employ different enrichment media. STEC and S. Typhimurium were detected after 12 and 18 h and their presence was confirmed by colony isolation. Conclusions Both methods were able to detect STEC in ground beef after 12 h of enrichment in samples inoculated with low levels of the pathogen. STEC and S. Typhimurium can be detected and isolated in co‐inoculated ground beef samples. Significance and Impact of the Study The IQ‐Check methods are comparable to the MLG methods for detection of STEC and simultaneous detection of STEC and S. Typhimurium in seeded ground beef after a short enrichment time, thus the IQ‐Check method can be useful for the food industry for rapid detection of these pathogens.