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Microbial diversity and biomarker analysis of modern freshwater microbialites from Laguna Bacalar, Mexico
Laguna Bacalar is a sulfate‐rich freshwater lake on the Yucatan Peninsula that hosts large microbialites. High sulfate concentrations distinguish Laguna Bacalar from other freshwater microbialite sites such as Pavilion Lake and Alchichica, Mexico, as well as from other aqueous features on the Yucata...
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Published in: | Geobiology 2018-05, Vol.16 (3), p.319-337 |
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description | Laguna Bacalar is a sulfate‐rich freshwater lake on the Yucatan Peninsula that hosts large microbialites. High sulfate concentrations distinguish Laguna Bacalar from other freshwater microbialite sites such as Pavilion Lake and Alchichica, Mexico, as well as from other aqueous features on the Yucatan Peninsula. While cyanobacterial populations have been described here previously, this study offers a more complete characterization of the microbial populations and corresponding biogeochemical cycling using a three‐pronged geobiological approach of microscopy, high‐throughput DNA sequencing, and lipid biomarker analyses. We identify and compare diverse microbial communities of Alphaproteobacteria, Deltaproteobacteria, and Gammaproteobacteria that vary with location along a bank‐to‐bank transect across the lake, within microbialites, and within a neighboring mangrove root agglomeration. In particular, sulfate‐reducing bacteria are extremely common and diverse, constituting 7%–19% of phylogenetic diversity within the microbialites, and are hypothesized to significantly influence carbonate precipitation. In contrast, Cyanobacteria account for less than 1% of phylogenetic diversity. The distribution of lipid biomarkers reflects these changes in microbial ecology, providing meaningful biosignatures for the microbes in this system. Polysaturated short‐chain fatty acids characteristic of cyanobacteria account for |
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B. ; Beddows, P. A. ; Flynn, T. M. ; Osburn, M. R.</creator><creatorcontrib>Johnson, D. B. ; Beddows, P. A. ; Flynn, T. M. ; Osburn, M. R. ; Argonne National Lab. (ANL), Argonne, IL (United States)</creatorcontrib><description>Laguna Bacalar is a sulfate‐rich freshwater lake on the Yucatan Peninsula that hosts large microbialites. High sulfate concentrations distinguish Laguna Bacalar from other freshwater microbialite sites such as Pavilion Lake and Alchichica, Mexico, as well as from other aqueous features on the Yucatan Peninsula. While cyanobacterial populations have been described here previously, this study offers a more complete characterization of the microbial populations and corresponding biogeochemical cycling using a three‐pronged geobiological approach of microscopy, high‐throughput DNA sequencing, and lipid biomarker analyses. We identify and compare diverse microbial communities of Alphaproteobacteria, Deltaproteobacteria, and Gammaproteobacteria that vary with location along a bank‐to‐bank transect across the lake, within microbialites, and within a neighboring mangrove root agglomeration. In particular, sulfate‐reducing bacteria are extremely common and diverse, constituting 7%–19% of phylogenetic diversity within the microbialites, and are hypothesized to significantly influence carbonate precipitation. In contrast, Cyanobacteria account for less than 1% of phylogenetic diversity. The distribution of lipid biomarkers reflects these changes in microbial ecology, providing meaningful biosignatures for the microbes in this system. Polysaturated short‐chain fatty acids characteristic of cyanobacteria account for <3% of total abundance in Laguna Bacalar microbialites. By contrast, even short‐chain and monounsaturated short‐chain fatty acids attributable to both Cyanobacteria and many other organisms including types of Alphaproteobacteria and Gammaproteobacteria constitute 43%–69% and 17%–25%, respectively, of total abundance in microbialites. While cyanobacteria are the largest and most visible microbes within these microbialites and dominate the mangrove root agglomeration, it is clear that their smaller, metabolically diverse associates are responsible for significant biogeochemical cycling in this microbialite system.</description><identifier>ISSN: 1472-4677</identifier><identifier>EISSN: 1472-4669</identifier><identifier>DOI: 10.1111/gbi.12283</identifier><identifier>PMID: 29656514</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Abundance ; Agglomeration ; Alphaproteobacteria ; Bacteria ; BASIC BIOLOGICAL SCIENCES ; Biogeochemical cycles ; Biogeochemistry ; Biomarkers ; Carbonates ; Cyanobacteria ; DNA ; DNA sequences ; DNA sequencing ; Fatty acids ; Freshwater ; Freshwater lakes ; Gammaproteobacteria ; GEOSCIENCES ; Inland water environment ; Laguna Bacalar ; lipid biomarkers ; Lipids ; Mangroves ; Microbial activity ; microbialites ; Microscopy ; Phylogenetics ; Phylogeny ; sulfate reducing bacteria ; Sulfates</subject><ispartof>Geobiology, 2018-05, Vol.16 (3), p.319-337</ispartof><rights>2018 John Wiley & Sons Ltd</rights><rights>2018 John Wiley & Sons Ltd.</rights><rights>Copyright © 2018 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4463-bf4dc36662c45c90c7db90bd42a5e36c60e196db31a5582179b2bdbfcbdb08393</citedby><cites>FETCH-LOGICAL-c4463-bf4dc36662c45c90c7db90bd42a5e36c60e196db31a5582179b2bdbfcbdb08393</cites><orcidid>0000-0001-9180-559X ; 0000-0002-1838-8942 ; 0000-0002-5482-5329</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29656514$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/servlets/purl/1461427$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Johnson, D. B.</creatorcontrib><creatorcontrib>Beddows, P. A.</creatorcontrib><creatorcontrib>Flynn, T. M.</creatorcontrib><creatorcontrib>Osburn, M. R.</creatorcontrib><creatorcontrib>Argonne National Lab. (ANL), Argonne, IL (United States)</creatorcontrib><title>Microbial diversity and biomarker analysis of modern freshwater microbialites from Laguna Bacalar, Mexico</title><title>Geobiology</title><addtitle>Geobiology</addtitle><description>Laguna Bacalar is a sulfate‐rich freshwater lake on the Yucatan Peninsula that hosts large microbialites. High sulfate concentrations distinguish Laguna Bacalar from other freshwater microbialite sites such as Pavilion Lake and Alchichica, Mexico, as well as from other aqueous features on the Yucatan Peninsula. While cyanobacterial populations have been described here previously, this study offers a more complete characterization of the microbial populations and corresponding biogeochemical cycling using a three‐pronged geobiological approach of microscopy, high‐throughput DNA sequencing, and lipid biomarker analyses. We identify and compare diverse microbial communities of Alphaproteobacteria, Deltaproteobacteria, and Gammaproteobacteria that vary with location along a bank‐to‐bank transect across the lake, within microbialites, and within a neighboring mangrove root agglomeration. In particular, sulfate‐reducing bacteria are extremely common and diverse, constituting 7%–19% of phylogenetic diversity within the microbialites, and are hypothesized to significantly influence carbonate precipitation. In contrast, Cyanobacteria account for less than 1% of phylogenetic diversity. The distribution of lipid biomarkers reflects these changes in microbial ecology, providing meaningful biosignatures for the microbes in this system. Polysaturated short‐chain fatty acids characteristic of cyanobacteria account for <3% of total abundance in Laguna Bacalar microbialites. By contrast, even short‐chain and monounsaturated short‐chain fatty acids attributable to both Cyanobacteria and many other organisms including types of Alphaproteobacteria and Gammaproteobacteria constitute 43%–69% and 17%–25%, respectively, of total abundance in microbialites. While cyanobacteria are the largest and most visible microbes within these microbialites and dominate the mangrove root agglomeration, it is clear that their smaller, metabolically diverse associates are responsible for significant biogeochemical cycling in this microbialite system.</description><subject>Abundance</subject><subject>Agglomeration</subject><subject>Alphaproteobacteria</subject><subject>Bacteria</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biogeochemical cycles</subject><subject>Biogeochemistry</subject><subject>Biomarkers</subject><subject>Carbonates</subject><subject>Cyanobacteria</subject><subject>DNA</subject><subject>DNA sequences</subject><subject>DNA sequencing</subject><subject>Fatty acids</subject><subject>Freshwater</subject><subject>Freshwater lakes</subject><subject>Gammaproteobacteria</subject><subject>GEOSCIENCES</subject><subject>Inland water environment</subject><subject>Laguna Bacalar</subject><subject>lipid biomarkers</subject><subject>Lipids</subject><subject>Mangroves</subject><subject>Microbial activity</subject><subject>microbialites</subject><subject>Microscopy</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>sulfate reducing bacteria</subject><subject>Sulfates</subject><issn>1472-4677</issn><issn>1472-4669</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kUsvBTEYhhshLoeFPyANGxKH3mdmibglR2xYN70NZWZKO4Pz7_UYLCS-Rb8239O3zfsCsI3REc51_KD9ESakpEtgHbOCTJkQ1fLvvijWwEZKTwgRxileBWukElxwzNaBv_EmBu1VA61_czH5fg5VZ6H2oVXx2cV8Us08-QRDDdtgXexgHV16fFd9nrY_933vUh6EFs7Uw9ApeKqMalQ8hDfuw5uwCVZq1SS39d0n4P7i_O7sajq7vbw-O5lNDWOCTnXNrKFCCGIYNxUyhdUV0pYRxR0VRiCHK2E1xYrzkuCi0kRbXZu8oJJWdAJ2R92Qei-Tyf8yjyZ0nTO9xExgRooM7Y_QSwyvg0u9bH0yrmlU58KQJEGElwjxkmZ07w_6FIaYPRmpChdELF49GKnsRkrR1fIl-mzgXGIkFyHJHJL8CimzO9-Kg26d_SV_UsnA8Qi8-8bN_1eSl6fXo-QnZCqbLw</recordid><startdate>201805</startdate><enddate>201805</enddate><creator>Johnson, D. B.</creator><creator>Beddows, P. A.</creator><creator>Flynn, T. M.</creator><creator>Osburn, M. R.</creator><general>Wiley Subscription Services, Inc</general><general>Wiliey</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OIOZB</scope><scope>OTOTI</scope><orcidid>https://orcid.org/0000-0001-9180-559X</orcidid><orcidid>https://orcid.org/0000-0002-1838-8942</orcidid><orcidid>https://orcid.org/0000-0002-5482-5329</orcidid></search><sort><creationdate>201805</creationdate><title>Microbial diversity and biomarker analysis of modern freshwater microbialites from Laguna Bacalar, Mexico</title><author>Johnson, D. B. ; Beddows, P. A. ; Flynn, T. M. ; Osburn, M. R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4463-bf4dc36662c45c90c7db90bd42a5e36c60e196db31a5582179b2bdbfcbdb08393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Abundance</topic><topic>Agglomeration</topic><topic>Alphaproteobacteria</topic><topic>Bacteria</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biogeochemical cycles</topic><topic>Biogeochemistry</topic><topic>Biomarkers</topic><topic>Carbonates</topic><topic>Cyanobacteria</topic><topic>DNA</topic><topic>DNA sequences</topic><topic>DNA sequencing</topic><topic>Fatty acids</topic><topic>Freshwater</topic><topic>Freshwater lakes</topic><topic>Gammaproteobacteria</topic><topic>GEOSCIENCES</topic><topic>Inland water environment</topic><topic>Laguna Bacalar</topic><topic>lipid biomarkers</topic><topic>Lipids</topic><topic>Mangroves</topic><topic>Microbial activity</topic><topic>microbialites</topic><topic>Microscopy</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>sulfate reducing bacteria</topic><topic>Sulfates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johnson, D. B.</creatorcontrib><creatorcontrib>Beddows, P. A.</creatorcontrib><creatorcontrib>Flynn, T. M.</creatorcontrib><creatorcontrib>Osburn, M. R.</creatorcontrib><creatorcontrib>Argonne National Lab. 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(ANL), Argonne, IL (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbial diversity and biomarker analysis of modern freshwater microbialites from Laguna Bacalar, Mexico</atitle><jtitle>Geobiology</jtitle><addtitle>Geobiology</addtitle><date>2018-05</date><risdate>2018</risdate><volume>16</volume><issue>3</issue><spage>319</spage><epage>337</epage><pages>319-337</pages><issn>1472-4677</issn><eissn>1472-4669</eissn><abstract>Laguna Bacalar is a sulfate‐rich freshwater lake on the Yucatan Peninsula that hosts large microbialites. High sulfate concentrations distinguish Laguna Bacalar from other freshwater microbialite sites such as Pavilion Lake and Alchichica, Mexico, as well as from other aqueous features on the Yucatan Peninsula. While cyanobacterial populations have been described here previously, this study offers a more complete characterization of the microbial populations and corresponding biogeochemical cycling using a three‐pronged geobiological approach of microscopy, high‐throughput DNA sequencing, and lipid biomarker analyses. We identify and compare diverse microbial communities of Alphaproteobacteria, Deltaproteobacteria, and Gammaproteobacteria that vary with location along a bank‐to‐bank transect across the lake, within microbialites, and within a neighboring mangrove root agglomeration. In particular, sulfate‐reducing bacteria are extremely common and diverse, constituting 7%–19% of phylogenetic diversity within the microbialites, and are hypothesized to significantly influence carbonate precipitation. In contrast, Cyanobacteria account for less than 1% of phylogenetic diversity. The distribution of lipid biomarkers reflects these changes in microbial ecology, providing meaningful biosignatures for the microbes in this system. Polysaturated short‐chain fatty acids characteristic of cyanobacteria account for <3% of total abundance in Laguna Bacalar microbialites. By contrast, even short‐chain and monounsaturated short‐chain fatty acids attributable to both Cyanobacteria and many other organisms including types of Alphaproteobacteria and Gammaproteobacteria constitute 43%–69% and 17%–25%, respectively, of total abundance in microbialites. While cyanobacteria are the largest and most visible microbes within these microbialites and dominate the mangrove root agglomeration, it is clear that their smaller, metabolically diverse associates are responsible for significant biogeochemical cycling in this microbialite system.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29656514</pmid><doi>10.1111/gbi.12283</doi><tpages>19</tpages><orcidid>https://orcid.org/0000-0001-9180-559X</orcidid><orcidid>https://orcid.org/0000-0002-1838-8942</orcidid><orcidid>https://orcid.org/0000-0002-5482-5329</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Abundance Agglomeration Alphaproteobacteria Bacteria BASIC BIOLOGICAL SCIENCES Biogeochemical cycles Biogeochemistry Biomarkers Carbonates Cyanobacteria DNA DNA sequences DNA sequencing Fatty acids Freshwater Freshwater lakes Gammaproteobacteria GEOSCIENCES Inland water environment Laguna Bacalar lipid biomarkers Lipids Mangroves Microbial activity microbialites Microscopy Phylogenetics Phylogeny sulfate reducing bacteria Sulfates |
title | Microbial diversity and biomarker analysis of modern freshwater microbialites from Laguna Bacalar, Mexico |
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