Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom

Oceanic harmful algal blooms ofPseudo-nitzschiadiatoms produce the potent mammalian neurotoxin domoic acid (DA). Despite decades of research, the molecular basis for its biosynthesis is not known. By using growth conditions known to induce DA production inPseudo-nitzschia multiseries, we implemented...

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Published in:Science (American Association for the Advancement of Science) 2018-09, Vol.361 (6409)
Main Authors: Brunson, John K., McKinnie, Shaun M. K., Chekan, Jonathan R., McCrow, John P., Miles, Zachary D., Bertrand, Erin M., Bielinski, Vincent A., Luhavaya, Hanna, Oborník, Miroslav, Smith, G. Jason, Hutchins, David A., Allen, Andrew E., Moore, Bradley S.
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container_title Science (American Association for the Advancement of Science)
container_volume 361
creator Brunson, John K.
McKinnie, Shaun M. K.
Chekan, Jonathan R.
McCrow, John P.
Miles, Zachary D.
Bertrand, Erin M.
Bielinski, Vincent A.
Luhavaya, Hanna
Oborník, Miroslav
Smith, G. Jason
Hutchins, David A.
Allen, Andrew E.
Moore, Bradley S.
description Oceanic harmful algal blooms ofPseudo-nitzschiadiatoms produce the potent mammalian neurotoxin domoic acid (DA). Despite decades of research, the molecular basis for its biosynthesis is not known. By using growth conditions known to induce DA production inPseudo-nitzschia multiseries, we implemented transcriptome sequencing in order to identify DA biosynthesis genes that colocalize in a genomic four-gene cluster. We biochemically investigated the recombinant DA biosynthetic enzymes and linked their mechanisms to the construction of DA’s diagnostic pyrrolidine skeleton, establishing a model for DA biosynthesis. Knowledge of the genetic basis for toxin production provides an orthogonal approach to bloom monitoring and enables study of environmental factors that drive oceanic DA production.
doi_str_mv 10.1126/science.aau0382
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title Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom
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