Lanthanide-Binding Tags for 3D X‑ray Imaging of Proteins in Cells at Nanoscale Resolution

We report the application of lanthanide-binding tags (LBTs) for two- and three-dimensional X-ray imaging of individual proteins in cells with a sub-15 nm beam. The method combines encoded LBTs, which are tags of minimal size (ca. 15–20 amino acids) affording high-affinity lanthanide ion binding, and...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2020-02, Vol.142 (5), p.2145-2149
Main Authors: Victor, Tiffany W, O’Toole, Katherine H, Easthon, Lindsey M, Ge, Mingyuan, Smith, Randy J, Huang, Xiaojing, Yan, Hanfei, Chu, Yong S, Chen, Si, Gursoy, Doga, Ralle, Martina, Imperiali, Barbara, Allen, Karen N, Miller, Lisa M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
BASIC BIOLOGICAL SCIENCES
Electrochemical cells
Fluorescence
Imaging, Three-Dimensional - methods
Lanthanides
Lanthanoid Series Elements - metabolism
Protein Binding
Proteins - chemistry
Spectrometry, X-Ray Emission - methods
X-raysPeptides and proteins
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Summary:We report the application of lanthanide-binding tags (LBTs) for two- and three-dimensional X-ray imaging of individual proteins in cells with a sub-15 nm beam. The method combines encoded LBTs, which are tags of minimal size (ca. 15–20 amino acids) affording high-affinity lanthanide ion binding, and X-ray fluorescence microscopy (XFM). This approach enables visualization of LBT-tagged proteins while simultaneously measuring the elemental distribution in cells at a spatial resolution necessary for visualizing cell membranes and eukaryotic subcellular organelles.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.9b11571