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Lanthanide-Binding Tags for 3D X‑ray Imaging of Proteins in Cells at Nanoscale Resolution
We report the application of lanthanide-binding tags (LBTs) for two- and three-dimensional X-ray imaging of individual proteins in cells with a sub-15 nm beam. The method combines encoded LBTs, which are tags of minimal size (ca. 15–20 amino acids) affording high-affinity lanthanide ion binding, and...
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| Published in: | Journal of the American Chemical Society 2020-02, Vol.142 (5), p.2145-2149 |
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| Main Authors: | , , , , , , , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-a389t-bf94f16f9097bc76b8f0651595f196180a40b5f1c268153a993b65422fbf7cd3 |
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| cites | cdi_FETCH-LOGICAL-a389t-bf94f16f9097bc76b8f0651595f196180a40b5f1c268153a993b65422fbf7cd3 |
| container_end_page | 2149 |
| container_issue | 5 |
| container_start_page | 2145 |
| container_title | Journal of the American Chemical Society |
| container_volume | 142 |
| creator | Victor, Tiffany W O’Toole, Katherine H Easthon, Lindsey M Ge, Mingyuan Smith, Randy J Huang, Xiaojing Yan, Hanfei Chu, Yong S Chen, Si Gursoy, Doga Ralle, Martina Imperiali, Barbara Allen, Karen N Miller, Lisa M |
| description | We report the application of lanthanide-binding tags (LBTs) for two- and three-dimensional X-ray imaging of individual proteins in cells with a sub-15 nm beam. The method combines encoded LBTs, which are tags of minimal size (ca. 15–20 amino acids) affording high-affinity lanthanide ion binding, and X-ray fluorescence microscopy (XFM). This approach enables visualization of LBT-tagged proteins while simultaneously measuring the elemental distribution in cells at a spatial resolution necessary for visualizing cell membranes and eukaryotic subcellular organelles. |
| doi_str_mv | 10.1021/jacs.9b11571 |
| format | article |
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| fulltext | fulltext |
| identifier | ISSN: 0002-7863 |
| ispartof | Journal of the American Chemical Society, 2020-02, Vol.142 (5), p.2145-2149 |
| issn | 0002-7863 1520-5126 |
| language | eng |
| recordid | cdi_osti_scitechconnect_1604321 |
| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Amino Acid Sequence BASIC BIOLOGICAL SCIENCES Electrochemical cells Fluorescence Imaging, Three-Dimensional - methods Lanthanides Lanthanoid Series Elements - metabolism Protein Binding Proteins - chemistry Spectrometry, X-Ray Emission - methods X-raysPeptides and proteins |
| title | Lanthanide-Binding Tags for 3D X‑ray Imaging of Proteins in Cells at Nanoscale Resolution |
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