Corticosterone in American alligator (Alligator mississippiensis) tail scutes: Evaluating the feasibility of using unconventional samples for investigating environmental stressors

• Published in: General and comparative endocrinology 2018-11, Vol.268 (C), p.7-13
• Main Authors: Hamilton, Matthew T., Finger, John W., Elsey, Ruth M., Mastromonaco, Gabriela F., Tuberville, Tracey D.
• Format: Article
• Language: English
• Subjects: Alligators and Crocodiles; Animals; Body condition index; Chronic stress; Corticosterone - blood; Crocodilians; Ecotoxicology - trends; Endocrinology & Metabolism; Enzyme immunoassay; Feasibility Studies; Glucocorticoids; Glucocorticoids - blood; Tissue
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2018.07.008

•Modified extraction techniques have allowed for tissue glucocorticoid quantification.•We reliably extracted corticosterone (CORT) from American alligator scute tissue.•Scute CORT levels increased less than plasma CORT after a two-hour stressor.•Body condition and individual scutes may influence CORT concentrations.•Crocodilian scute CORT may provide insight into stress under controlled conditions. Baseline plasma corticosterone (CORT) concentrations have been widely used to investigate the effects of stressors in wild and captive crocodilians. However, collecting baseline plasma CORT samples from wild crocodilians may be particularly difficult due to the capture and handling protocols used for large individuals. Thus, it may prove beneficial to use recently modified techniques for extracting CORT deposited in keratinized and non-keratinized tissues to better quantify the effects of long-term stress in crocodilians. In this study, we investigated the feasibility of using American alligator (Alligator mississippiensis) tail scute tissues to quantify CORT by collecting blood and tail scutes from 40 alligators before and after a short-term handling stressor. The objective of the current study was to better understand CORT deposition in crocodilian scutes and whether short-term increases in CORT could be detected. We found that CORT can be reliably extracted from alligator scute tissue and quantified using a commercially available enzyme immunoassay. However, there was a significant increase in scute CORT concentrations following an alligator being exposed to a short-term stressor (p = 0.017), although the magnitude of change was less than observed in plasma samples from the same individuals (p = 0.002). Furthermore, our results indicate that there was a significant effect of body condition on an alligator’s post-stressor CORT concentration (p = 0.02). While our study is among the first to experimentally examine the usefulness of tissue CORT in crocodilians, a combination of field and laboratory experiments are needed to better understand deposition rates of CORT in scute tissues and to further validate the usefulness of tissue glucocorticoids for evaluating the effects of stress.