Ultracytochemical Observations of Glycogen Synthesis and Storage during Infradian Growth of Tetrahymena pyriformis

In the course of study of some molecular properties of the ciliated protozoan Tetrahymena pyriformis was a function of its mode of growth, conventional thin sections were examined in the electron microscope. As the cells progressed in their growth from the ultradian or fast exponential mode to the i...

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Bibliographic Details
Published in:The Journal of cell biology 1973-07, Vol.58 (1), p.240-244
Main Authors: Sutherland, Alexander, Antipa, Gregory A., Ehret, Charles F.
Format: Article
Language:English
Subjects:
Animals
BASIC BIOLOGICAL SCIENCES
Brief Notes
Cell growth
Cells
Centrioles
Chromosomes
Cilia
Circadian Rhythm
Cultured cells
Evaluation Studies as Topic
Glycogen
Glycogen - biosynthesis
Histocytochemistry
Histones
Microscopy, Electron
Oviducts
Tetrahymena pyriformis - cytology
Tetrahymena pyriformis - growth & development
Tetrahymena pyriformis - metabolism
Yeasts
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Summary:In the course of study of some molecular properties of the ciliated protozoan Tetrahymena pyriformis was a function of its mode of growth, conventional thin sections were examined in the electron microscope. As the cells progressed in their growth from the ultradian or fast exponential mode to the infrathan or slow exponential (quasi-stationary) mode, their ultrastructural appearance was that of cells becoming progressively emptied of their normal endoplasmic constituents . The major portion of the endoplasm had no conspicuously evident contents other than the macronucleus . Suspecting the synthesis and storage of glycogen not only because of the dramatic gross rise in assays of anthrone-positive material in our own' and previous work, but also because of the abundant supply of D-glucose in the medium, we applied an ultracytochemical technique for the demonstration of glycogen particles, the periodic acid/ thiosemicarbazide/silver protein method of Thiery, to the sections.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.58.1.240