Long-term organic fertilization increased antibiotic resistome in phyllosphere of maize

Phyllosphere contains various microorganisms that may harbor diverse antibiotic resistance genes (ARGs). However, we know little about the composition of antibiotic resistome and the factors influencing the diversity and abundance of ARGs in the phyllosphere. In this study, 16S rRNA gene amplicon se...

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Bibliographic Details
Published in:The Science of the total environment 2018-12, Vol.645 (C), p.1230-1237
Main Authors: Chen, Qing-Lin, An, Xin-Li, Zheng, Bang-Xiao, Ma, Yi-Bing, Su, Jian-Qiang
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents
Antibiotic resistance
Chicken manure
Drug Resistance, Microbial - genetics
Genes, Bacterial
Leaf-microbiome
Phyllosphere
RNA, Ribosomal, 16S
Sludge
Soil
Zea mays - physiology
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Summary:Phyllosphere contains various microorganisms that may harbor diverse antibiotic resistance genes (ARGs). However, we know little about the composition of antibiotic resistome and the factors influencing the diversity and abundance of ARGs in the phyllosphere. In this study, 16S rRNA gene amplicon sequencing and high-throughput quantitative PCR approaches were employed to investigate the effects of long-term (over 10 years) organic fertilization on the phyllosphere bacterial communities and antibiotic resistome. Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes dominated in the phyllosphere bacterial communities. Long-term application of sewage sludge and chicken manure altered the phyllosphere bacterial community composition, with a remarkable decrease in bacterial alpha-diversity. A total of 124 unique ARGs were detected in the phyllosphere. The application of sewage sludge and chicken manure significantly increased the abundance of ARGs, with a maximum 2638-fold enrichment. Variation partitioning analysis (VPA) together with network analysis indicated that the profile of ARGs is strongly correlated with bacterial community compositions. These results improve the knowledge about the diversity of plant-associated antibiotic resistome and factors influencing the profile of ARGs in the phyllosphere. [Display omitted] •Sewage sludge and manure amendment increased the occurrence and abundance of ARGs in phyllosphere.•Bacterial community change was the major drives in shaping ARGs in the phyllosphere.•Soil may serve as an antibiotic resistome reservoir for phyllosphere.
ISSN:0048-9697
1879-1026
DOI:10.1016/j.scitotenv.2018.07.260