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myo-inositol facilitates salinity tolerance by modulating multiple physiological functions in the turbot Scophthalmus maximus

As a B-complex vitamin that is a compatible organic osmolyte and a component of phosphatidylinositol in biological cell membranes, myo-inositol plays vital roles in responses to salinity stress. A significant increase in the myo-inositol content of the gill of turbot exposed to salinity stress was d...

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Published in:Aquaculture 2020-10, Vol.527 (C), p.735451, Article 735451
Main Authors: Cui, Wenxiao, Ma, Aijun, Huang, Zhihui, Liu, Zhifeng, Yang, Kai, Zhang, Wei
Format: Article
Language:English
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Summary:As a B-complex vitamin that is a compatible organic osmolyte and a component of phosphatidylinositol in biological cell membranes, myo-inositol plays vital roles in responses to salinity stress. A significant increase in the myo-inositol content of the gill of turbot exposed to salinity stress was detected using the enzyme-linked immunosorbent assay. myo-inositol supplementation provided by immersion or inclusion in the diet significantly extended survival under salinity stress, with the exception of immersion at salinity 0. Transcriptome data indicated that myo-inositol increased physiological capacities related to the steroid biosynthetic process, steroid metabolic process, circadian rhythm, tryptophan metabolism, metabolism of xenobiotics by cytochrome P450, oxidoreductase activity, iron ion binding, and heme binding in turbot. Furthermore, the qPCR results showed that myo-inositol strengthened osmotic regulation, as represented by the expression of ion-channel genes of turbot under salinity stress by stimulating the activities of steroid reductase and antioxidase, modulating immune function, and inhibiting the cell cycle and energy metabolism. GO and KEGG enrichment analyses of differentially expressed genes, PPI analysis, and qPCR data showed that signalling pathways associated with steroids including the steroid biosynthetic process, steroid metabolic process, and steroid hormone biosynthesis, mediated by myo-inositol occupy a central place in osmoregulation in turbot. •The effects of myo-inositol on the turbot response to salinity stress were investigated in multiple ways.•The regulatory mechanisms of myo-inositol in fish were explored for the first time at the transcriptome level.•myo-inositol increases the capacity of salinity tolerance mainly by modulating physiological functions related to steroids.
ISSN:0044-8486
1873-5622
DOI:10.1016/j.aquaculture.2020.735451