Expression profiling of the Kdm genes in scallop Patinopecten yessoensis suggests involvement of histone demethylation in regulation of early development and gametogenesis

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2020-06, Vol.243-244 (C), p.110434-110434, Article 110434
• Main Authors: Guo, Zhenyi, Zhang, Lijing, Li, Yajuan, Wu, Shaoxuan, Wang, Shi, Zhang, Lingling, Bao, Zhenmin
• Format: Article
• Language: English
• Subjects: Early development; Gametogenesis; Histone demethylation; Kdm; Patinopecten yessoensis
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2020.110434

Histone demethylation modification is an important means of gene expression regulation and is widely involved in biological processes such as animal reproduction and development. Histone lysine demethylases (Kdm) plays an important role in the demethylation of histones. To understand the role of histone demethylation in scallops, we identified the Kdm gene family of the Yesso scallop Patinopecten yessoensis, and analyzed its expression during the gonad and early development. The results showed that the P. yessoensis has a complete Kdm family including seventeen members that belong to sixteen subfamilies (Hif1an, Hspbap1, Jarid2, Jmjd4, Jmjd6, Jmjd7, Jmjd8, Kdm1, Kdm2, Kdm3, Kdm4, Kdm5, Kdm6, Kdm7, Kdm8 and Kdm9). The Kdm genes showed five different expression patterns in the early development of scallop, with some of them (e.g. Jmjd7, Jmjd8 and Kdm8) being highly expressed in only one or two stage and the others (e.g. Kdm1A, Kdm9, Jmjd4 and Jmjd6) in several consecutive stages. During gonadal development, the Kdm genes also display various expression patterns. Some genes (e.g. Kdm2, Kdm4 and Jmjd7) display preferential expression in the testis, and the others have no obvious sex bias but show stage preference (resting, proliferative, growing or maturation stage). These results suggest that various histone demethylation modifications (e.g. H3K4, H3K9 and H3K27) may participate in the regulation of gametogenesis and early development of Yesso scallop. It will facilitate a better understanding of the epigenetic contributions to mollusk development. [Display omitted] •The Yesso scallop has a complete and conservative Kdm family including 17 members.•The Kdm genes display diverse expression patterns during gonad and early development.•Various histone demethylation modifications may contribute to scallop development.