Panax quinquefolius (North American ginseng) cell suspension culture as a source of bioactive polysaccharides: Immunostimulatory activity and characterization of a neutral polysaccharide AGC1

In this study, we propose the use of a plant tissue culture-based system for the production of polysaccharides with consistent chemical characteristics and reduced endotoxin content. Polysaccharides were isolated from suspension cultures of Panax quinquefolius (American ginseng), a widely used medic...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2019-10, Vol.139 (C), p.221-232
Main Authors: Ghosh, Rajarshi, Smith, Shannon A., Nwangwa, Evidence E., Arivett, Brock A., Bryant, Daniel L., Fuller, Matthew L., Hayes, Devyn, Bowling, J. Logan, Nelson, David E., DuBois, John D., Altman, Elliot, Kline, Paul C., Farone, Anthony L.
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Culture Media, Conditioned - chemistry
Culture Media, Conditioned - pharmacology
Cytokines - metabolism
Immunologic Factors - chemistry
Immunologic Factors - pharmacology
Immunomodulation
Immunomodulation - drug effects
Macrophages - drug effects
Macrophages - immunology
Macrophages - metabolism
Mice
Molecular Weight
Nitric Oxide - metabolism
NOS2 reporter
Panax - chemistry
Panax - cytology
Panax - metabolism
Phytochemicals - chemistry
Phytochemicals - isolation & purification
Phytochemicals - pharmacology
Plant Extracts - chemistry
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
RAW 264.7 Cells
Suspension culture polysaccharides
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, we propose the use of a plant tissue culture-based system for the production of polysaccharides with consistent chemical characteristics and reduced endotoxin content. Polysaccharides were isolated from suspension cultures of Panax quinquefolius (American ginseng), a widely used medicinal herb. A neutral fraction, AGC1, purified by anion exchange and size exclusion chromatography, displayed immunostimulatory activity in vitro and ex vivo. AGC1 (average molecular weight: 5.2kDa) was predominantly composed of galactose (>60%) along with the presence of several other neutral sugars such as arabinose, xylose, glucose, mannose and rhamnose in minor amounts. The major glycosidic linkages were found to be 3-Galp (48.5%), 3,6-Galp (10.2%), t-Galp (5.2%), 6-Galp (4.4%), 4-Glcp (5.7%), 4-Arap/5-Araf (4.0%) and t-Araf (4.5%). AGC1 significantly (p
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2019.07.215