Improving biomass and lipid yields of Desmodesmus armatus and Chlorella vulgaris through mutagenesis and high-throughput screening

• Published in: Biomass & bioenergy 2020-09, Vol.142 (105755)
• Main Authors: Smalley, Tressa, Fields, Francis J., Berndt, Anthony J. E., Ostrand, Joseph T., Heredia, Vanessa, Mayfield, Stephen P.
• Format: Article
• Language: English
• Subjects: 09 BIOMASS FUELS; 60 APPLIED LIFE SCIENCES; BASIC BIOLOGICAL SCIENCES; biofuel; FACS; Microalgae; mutagenesis; Nile; red; strain improvement
• ISSN: 0961-9534; 1873-2909

Advancement of microalgae for use as biofuel feedstock depends on bioprospecting novel strains and then applying classical strain improvement techniques to enhance desirable traits. Here we successfully demonstrate this approach using two novel strains of green algae, identified as Desmodesmus armatus and Chlorella vulgaris, to improve biomass and lipid yields. UV irradiation was utilized to generate random mutations and the resulting populations were analyzed via flow cytometry to quantify lipid fluorescence while enriching for high lipid variants using fluorescence activated cell sorting. Secondary screening was performed via in situ fluorescence staining to identify the single highest lipid producing mutants from each strain. This approach produced a Desmodesmus armatus mutant that exhibited both increased biomass productivity and higher lipid content, which doubled lipid yield from the culture. The resulting Chlorella vulgaris mutant exhibited an increase in growth only, resulting in a 33% increase in lipid yield.